Overview
 | The CM20 incubation monitoring system provides quantitative data remotely—place the head and your cell cultures in the incubator, and the system will periodically scan it, count the number of cells, and determine confluency. The data can be wirelessly communicated from a computer workstation, so you can monitor your cultures’ progress without entering the clean room. *This product is not available in some areas Until March 2024, we will continue to sell while stocks last. CM20 is replaced with CM30. |
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 Application GalleryCM20 Incubation Monitoring System supports observation and data analysis of various cells and vessels. |
Meet the Olympus Provi CM20 Incubation Monitoring SystemBy automating the measurements which was previously done manually with AI, brings you efficient workflow and quantitative results | Related Videos |
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How to Start Your Cell Culture with the CM20 Monitor |
Related VideosSet Up the System | Related VideosSet Up a Project | Related VideosAnalyze Data |
Applied Technologies
Customize the Settings to Meet Your NeedsMonitoring pointsSet the monitoring points in the vessel and well plate anywhere you would like, as shown in the vessel on the right. By specifying the rows and columns, you can set multiple points at once. Focus positionFreely set the focus position for each monitoring point. Monitoring timeFreely set the start time and interval time. |
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Flat Design Accommodates Various VesselsOlympus’ epi-oblique optical system enables the CM20 incubation monitoring system to have a compact, flat design that accommodates most standard cell culture vessels. Simply place the culture vessel you normally use on CM20. |
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Cells Remain UndamagedWhen cell cultures are removed from the incubator to be checked, the temperature change, vibration, and potential exposure to contaminants can cause damage. With the CM20 monitoring system, you can keep track of your cell cultures' health while they remain safely in the incubator. |  |
Supports Multi-Well MicroplatesExpands compatibility with multi-well plates for up to 96 wells. Monitor and compare the culture status of multiple wells at the same time under different conditions. Supported multi-well plate formats include:
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Multipoint Cell Culture MonitoringThe system automatically scans multiple points in your culture vessel, providing periodic quantitative data about the health and confluency of your cultures. |
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| AI-Driven Automated Cell AnalysisBy training the system to distinguish between the cells and background using machine learning, it can automatically determine confluency. The reliability of the quantitative data is improved by using images of three confluent phases for the training. |
Reproducible, Comparable DataThe CM20 makes it easy to store and share detailed quantitative records of cell growth and health.
•Graph confluency data to look at cell counts and colony analysis in chronological order to see how they’ve changed over time
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Application Programming Interface (API)
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IQ/OQ Validation ServiceOur world-class support team can help you integrate the CM20 incubation monitoring system into your lab. IQ/OQ validation services are also available. |
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Need assistance? |
Specifications
CM20H: Incubation Monitoring Head |
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Installation environment
(inside the incubator) | Temperature: 37 °C (98.6 °F) + 0.3 °C (0.5 °F), humidity: 0—99% |
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| Applicable vessels | Petri dish (90 mm (3.54 in.), 100 mm (3.94 in.)) |
| Microplate (6 well, 12 well, 24 well, 48 well, 96 well) | |
| Flask (T25, T75, T80, T150, T175, T225) | |
| Multi-layer flask | |
| Optical performance |
Field of view (H × V): 2.84 mm × 2.13 mm (0.11 in. × 0.08 in.);
(image size per one shooting) |
| Image size: 1280 × 960 pixels | |
| Illumination wavelength: λ = 630 nm (LED) | |
| Illumination method: epi-oblique illumination | |
| Cable length | Approx. 4.5 m (14.8 ft) |
| Sterilization resistance | Autoclave sterilization (for vessel holder and sponge rubber only) |
| UV ray sterilization | |
| Hydrogen peroxide (H₂O₂) gas sterilization (CM20H only) | |
| Disinfection resistance | Peracetic acid disinfection (cold sterilant) |
| Alcohol disinfection | |
| Weight | Approx. 3 kg (6.6 lb) |
Incubation Monitoring Station |
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Installation environment
(outside the incubator) | Temperature: 10—35 °C (50—95 °F), Humidity: 10—80% |
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| Number of connectable CM20H | Max. 4 heads |
| HDD capacity | 4 TB |
Software |
| User management | 13 user licenses (max) |
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| Project setting | Project creation: new or load |
| Settings: standard or custom | |
| Culture conditions: vessel information, culture information etc. | |
| Cell analysis conditions: new or load | |
| Access authority: public or private | |
| Imaging interval: selection type | |
| Analysis | Cell analysis: cell confluency, cell count |
| iPS/ES cell analysis: colony confluency, colony count, colony size | |
| Data statistics: growth rate, doubling time | |
| Browsing | Image: entire area (tiling), fixed points |
| Analysis result: graph (time, passage) | |
| Export | Data export: image file, movie file* CSV file* *only for fixed points |
| Create report (PDF) |
Client PC (Recommended System Configuration for CM20 Software) |
| OS | Microsoft® Windows® 10 (64-bit) |
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| CPU | Intel® Core TM i3 (2.1 GHz) or more |
| RAM | 4 GB or more |
| HDD | Free space: 2 GB or more |
| Screen resolution | 1366 × 768 or more |
| Web browser | Google Chrome TM |
Operation Confirmed Incubator |
| Thermo Fisher Scientific | 51030388 |
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| Panasonic | MCO-170AICUVH-PJ |
| ASTEC | SMA-165DRS |
Incubator conditions required for use of the CM20 system:
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FAQ
FAQFunctions & SpecificationsCan the CM20 incubation monitoring head be installed in our current incubator?With its compact design (73 mm (2.9 in.) tall, 212 mm (8.3 in.) wide, and 360 mm (14.2 in.) long) and weighing only approximately 3 kg (6.6 lb), the CM20 fits easily in most standard-sized incubators. Is the head compatible with general cell culture containers?Yes. The CM20 is compatible with the following vessels:
What kinds of cells can the system observe?It’s designed to observe adherent cells, such as MSC and iPS cells. What can it measure?It estimates cell population and confluency from an acquired image. For samples that form a colony, it estimates the number of colonies and confluency. Will there be any damage to the cells?The system is designed to record quantitative data from inside an incubator, minimizing the chances of damaging your cell cultures. Can you observe fluorescence?It cannot observe fluorescence. We offer CKX53 inverted microscopes for fluorescence observation. How far can you remotely monitor?It depends on your router specifications and usage environment. If you want to extend the communication range, we recommend using a repeater. Can the CM20 incubation monitoring head be sterilized or disinfected?Yes. The head can be sterilized using:
Disinfected using:
SupportWhat is the support system after purchase?It depends on your region. Please contact your local sales representative. Is it possible to correspond when IQ / OQ validation is required?Yes. For details, please contact your local sales representative. Can the CM20 incubation monitoring system be connected to network?Yes. It is possible after building the connection environment at the customer's responsibility. However, we do not guarantee the network connection in the customer's environment. When connecting to the network, it is necessary to set the station PC. Learn more from here. |
Release Note
CM20 Version 1.2.8Improvements
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CM20H API Version 1.4.1 (Compatible with CM30H)Application Program Interface (API) which allows you to freely control the monitoring head CM20H/CM30H from your PC without the incubation monitoring station. Release note in the download package provides further details. New functions/Improvements Application Program Interface (API) which allows you to freely control the CM20H from your PC without the incubation monitoring station. Release note in the download package provides further details.
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CM20H API for Linux Version 1.4.1 (Compatible with CM30H)Application Program Interface (API) which allows you to freely control the monitoring station CM20H/CM30H from your PC or microcomputer board equipped with Linux without the incubation monitoring station. Release note in the download package provides further details. New functions/Improvements
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CM20 Version 1.2.7Improvements
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CM20 Version 1.2.6Improvement
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CM20 Version 1.2.5New applicable vessels
New function/improvement
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CM20H API Version 1.3.1New function/improvement Application Program Interface (API) which allows you to freely control the CM20H from your PC without the incubation monitoring station. Release note in the download package provides further details.
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CM20H API for Linux Version 1.3.1New function/improvement Application Program Interface (API) which allows you to freely control the CM20H from your PC or microcomputer board equipped with Linux without the incubation monitoring station. Release note in the download package provides further details.
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