Overview

	Fast, Accurate Cell Counting Streamlines the Cell Culture Process The cell counter’s software algorithm quickly provides accurate counting results for a variety of cell types by automatically de-clustering clumped cells, performing size- and roundness-based sorting, and identifying live/dead cells. The R1 cell counter can confirm cell counts prior to experimental data acquisition, quality check, passaging, or induction of differentiation. Select from default protocol settings for 18 of the 22 major cell types or customize a protocol to your specific cell type of interest. This product has been discontinued, check out our current product

Fast, Accurate Cell Counting Streamlines the Cell Culture Process

The cell counter’s software algorithm quickly provides accurate counting results for a variety of cell types by automatically de-clustering clumped cells, performing size- and roundness-based sorting, and identifying live/dead cells. The R1 cell counter can confirm cell counts prior to experimental data acquisition, quality check, passaging, or induction of differentiation. Select from default protocol settings for 18 of the 22 major cell types or customize a protocol to your specific cell type of interest.

This product has been discontinued, check out our current product

Accurate Cell Counting Accurately count clustered cells: The counter identifies single, clustered, live, and dead cells for counts with an accuracy similar to a hemocytometer. Exclude undesired particles with cell-size-based gates: Set the maximum and minimum cell sizes to filter for a given cell type. Accurately count small cells: The Live Cell Sensitivity function optimizes the recognition rate for cells less than 8 μm. Automated dilution calculator: Enter your desired concentrations, and the volume of cell suspension needed is automatically calculated. No trypan blue staining required: The Cell Counter can quickly count and present the total number of cells in a sample that has not been treated with trypan blue. The number of live cells can be estimated when the proportion of live/dead cells in the sample is known. Trypan blue concentration correction: The Auto Exposure Mode automatically corrects for variations in trypan blue concentration for stable count results.

Accurate Cell Counting

Accurately count clustered cells: The counter identifies single, clustered, live, and dead cells for counts with an accuracy similar to a hemocytometer.
Exclude undesired particles with cell-size-based gates: Set the maximum and minimum cell sizes to filter for a given cell type.
Accurately count small cells: The Live Cell Sensitivity function optimizes the recognition rate for cells less than 8 μm.
Automated dilution calculator: Enter your desired concentrations, and the volume of cell suspension needed is automatically calculated.
No trypan blue staining required: The Cell Counter can quickly count and present the total number of cells in a sample that has not been treated with trypan blue. The number of live cells can be estimated when the proportion of live/dead cells in the sample is known.
Trypan blue concentration correction: The Auto Exposure Mode automatically corrects for variations in trypan blue concentration for stable count results.

	Clear Cell Images with Liquid Lens Autofocusing The Cell Counter model R1 automatically focuses on cells using liquid lens technology, which works similar to how human eyes focus. The lens can autofocus in the Z-axis at the right position, producing more accurate and reproducible cell counting results.

Clear Cell Images with Liquid Lens Autofocusing

The Cell Counter model R1 automatically focuses on cells using liquid lens technology, which works similar to how human eyes focus. The lens can autofocus in the Z-axis at the right position, producing more accurate and reproducible cell counting results.

Efficient Cell Culture Data Management Easy Data File Sharing Store up to 1,000 counts Easily export your data as a CSV file Result and image data can be stored as a TIFF, an annotated TIFF, or a PDF report file Easily transfer data using the cell counter’s USB ports Comprehensive Report Results at a Glance No need to manually record detailed settings for each count Easily obtain report files containing the protocol used, the cell count results, the corresponding raw/analyzed cell images, and histograms Use review mode to view various reports on the touch screen at the same time, including the number of live/dead cells and average cell size

Efficient Cell Culture Data Management

Easy Data File Sharing

Store up to 1,000 counts
Easily export your data as a CSV file
Result and image data can be stored as a TIFF, an annotated TIFF, or a PDF report file
Easily transfer data using the cell counter’s USB ports

Comprehensive Report Results at a Glance

No need to manually record detailed settings for each count
Easily obtain report files containing the protocol used, the cell count results, the corresponding raw/analyzed cell images, and histograms
Use review mode to view various reports on the touch screen at the same time, including the number of live/dead cells and average cell size

	Custom Cell Counting Parameters Each detection parameter can be modified to fit the sample conditions. Up to 300 individual cell counting protocols can be saved and reloaded for use in routine procedures.

Custom Cell Counting Parameters

Each detection parameter can be modified to fit the sample conditions. Up to 300 individual cell counting protocols can be saved and reloaded for use in routine procedures.

User-Friendly Cell Counter Design

Portable with a Small Footprint

The handle on top of the counter makes it easy to transport, while its small footprint enables it to fit in tight spaces in your lab. A storage space in the back of the counter hides the AC adaptor and extension cord.

Convenient Touch Screen

The large touch screen at the base of the cell counter offers stability while making it simple to count cells, view data, and print the results.

Easy-to-Load Slides

It’s easy to add cells to the cell counter’s disposable slides through the loading slits. Two sets of cell counting are performed on one slide, making it easy to replicate runs.

Cell Counting Time ^*1	Less than 10 s (manual focusing)
Less than 15 s (auto focusing)
Cell Concentration Range	5 × 10⁴–1 × 10⁷ cells/mL
Cell Diameter Range	3–60 μm (recommended: 8–30 μm)
Output Information ^*2	Total/live/dead cell concentration
Total/live/dead cell number
Viability
Average cell size
Image Resolution	5 megapixels, color
Focusing Mode	Automatic/manual focusing with liquid lens
Adjustable Parameters	Size, roundness, dilution factor, noise reduction, declustering
Image Type	TIFF, annotated TIFF
Report Format	PDF
Count Data Storage	1,000 counts
Protocol Storage	300 protocols
Display	LCD touchscreen: 800 × 480, 17.8 cm (7 in.)
USB Port	3 ports
Thermal Printer	Built in
Dimensions (W × D × H)	195 mm × 237 mm × 272 mm (7.7 in. × 9.3 in. × 10.7 in.)
Weight	2.1 kg (4.6 lb) (without the external power adaptor)
Input Power	AC 100–240 V, 50/60 Hz, 1.2 A

Material	Polystyrene
Dimensions (W × D × H)	75 mm × 25 mm × 2.3 mm (3 in. × 1 in. × 0.09 in.)
Chamber Volume	10 μL
Quantity Per Box	100 counts (50 slides, 2 chambers per slide)

Protocol Parameters

Name： Description

FF hiPS：Feeder-free Human iPS cells (201B7 cell line)

Protocol

Results

Noise reduction: 6
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium

Total cell conc. (cells/ml): 1.50 x 10⁶
Live cell conc. (cells/ml): 1.38 x 10⁶
Dead cell conc. (cells/ml): 1.19 x 10⁵
Viability (%): 92.0
Average cell size (μm): 15.9

MEF：Murine embryonic Fibroblasts

Protocol

Results

Noise reduction: 7
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: None

Total cell conc. (cells/ml): 1.03 x 10⁶
Live cell conc. (cells/ml): 9.37 x 10⁵
Dead cell conc. (cells/ml): 9.72 x 10⁴
Viability (%): 90.6
Average cell size (μm): 19.6

A549： Human lung carcinoma

Protocol

Results

Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium

Total cell conc. (cells/ml): 1.09 x 10⁶
Live cell conc. (cells/ml): 1.07 x 10⁶
Dead cell conc. (cells/ml): 1.78 x 10⁴
Viability (%): 98.4
Average cell size (μm): 17.5

AsPC-1: Human pancreatic adenocarcinoma

Protocol

Results

Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: High

Total cell conc. (cells/ml): 7.51 x 10⁵
Live cell conc. (cells/ml): 7.19 x 10⁵
Dead cell conc. (cells/ml): 3.17 x 10⁴
Viability (%): 95.8
Average cell size (μm): 10.7

C6： Rat glioma

Protocol

Results

Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium

Total cell conc. (cells/ml): 1.31 x 10⁶
Live cell conc. (cells/ml): 1.22 x 10⁶
Dead cell conc. (cells/ml): 8.91 x 10⁴
Viability (%): 93.2
Average cell size (μm): 12.1

Capan-2： Human pancreatic adenocarcinoma

Protocol

Results

Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium

Total cell conc. (cells/ml): 1.04 x 10⁶
Live cell conc. (cells/ml): 1.00 x 10⁶
Dead cell conc. (cells/ml): 3.61 x 10⁴
Viability (%): 96.5
Average cell size (μm): 13.4

DU 145： Human prostate carcinoma

Protocol

Results

Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium

Total cell conc. (cells/ml): 1.86 x 10⁶
Live cell conc. (cells/ml): 1.80 x 10⁶
Dead cell conc. (cells/ml): 5.34 x 10⁴
Viability (%): 97.1
Average cell size (μm): 14.3

293-GFP： HEK293 stably expressing GFP

Protocol

Results

Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium

Total cell conc. (cells/ml): 1.84 x 10⁶
Live cell conc. (cells/ml): 1.82 x 10⁶
Dead cell conc. (cells/ml): 2.23 x 10⁴
Viability (%): 98.8
Average cell size (μm): 11.3

H1299： Human non-small cell lung carcinoma

Protocol

Results

Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium

Total cell conc. (cells/ml): 4.14 x 10⁵
Live cell conc. (cells/ml): 3.52 x 10⁵
Dead cell conc. (cells/ml): 6.23 x 10⁴
Viability (%): 84.9
Average cell size (μm): 19.5

H4： Human neuroglioma

Protocol

Results

Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium

Total cell conc. (cells/ml): 2.08 x 10⁵
Live cell conc. (cells/ml): 1.44 x 10⁵
Dead cell conc. (cells/ml): 6.32 x 10⁴
Viability (%): 69.6%
Average cell size (μm): 12.8

HCT116： Human colorectal carcinoma

Protocol

Results

Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium

Total cell conc. (cells/ml): 1.34 x 10⁶
Live cell conc. (cells/ml): 1.32 x 10⁶
Dead cell conc. (cells/ml): 1.81 x 10⁴
Viability (%): 98.7
Average cell size (μm): 12.2

HeLa： Human cervical carcinoma^*

Protocol

Results

Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium

Total cell conc. (cells/ml): 8.98 x 10⁵
Live cell conc. (cells/ml): 8.11 x 10⁵
Dead cell conc. (cells/ml): 8.69 x 10⁴
Viability (%): 90.3
Average cell size (μm): 17.6

Hep3B： Human hepatocellular carcinoma

Protocol

Results

Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium

Total cell conc. (cells/ml): 1.54 x 10⁶
Live cell conc. (cells/ml): 1.49 x 10⁶
Dead cell conc. (cells/ml): 5.87 x 10⁴
Viability (%): 96.2
Average cell size (μm): 14.9

HL-60： Human acute promyelocytic leukemia

Protocol

Results

Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium

Total cell conc. (cells/ml): 1.57 x 10⁶
Live cell conc. (cells/ml): 1.45 x 10⁶
Dead cell conc. (cells/ml): 1.23 x 10⁵
Viability (%): 92.2
Average cell size (μm): 14.9

Hs 578T： Human mammary carcinoma

Protocol

Results

Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium

Total cell conc. (cells/ml): 1.13 x 10⁶
Live cell conc. (cells/ml): 8.72 x 10⁵
Dead cell conc. (cells/ml): 2.62 x 10⁵
Viability (%): 76.9
Average cell size (μm): 16.5

MCF7： Human breast adenocarcinoma

Protocol

Results

Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium

Total cell conc. (cells/ml): 4.74 x 10⁵
Live cell conc. (cells/ml): 4.43 x 10⁵
Dead cell conc. (cells/ml): 4.07 x 10⁴
Viability (%): 91.4
Average cell size (μm): 16.2

NIH3T3： Mouse embryonic fibroblast

Protocol

Results

Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium

Total cell conc. (cells/ml): 1.52 x 10⁶
Live cell conc. (cells/ml): 1.33 x 10⁶
Dead cell conc. (cells/ml): 1.96 x 10⁵
Viability (%): 87.1
Average cell size (μm): 14.1

PANC-1： Human pancreatic ductal carcinoma

Protocol

Results

Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: High

Total cell conc. (cells/ml): 1.23 x 10⁶
Live cell conc. (cells/ml): 1.21 x 10⁵
Dead cell conc. (cells/ml): 2.26 x 10⁴
Viability (%): 98.2
Average cell size (μm): 15.3

PC-3： Human prostate adenocarcinoma

Protocol

Results

Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium

Total cell conc. (cells/ml): 1.36 x 10⁶
Live cell conc. (cells/ml): 1.33 x 10⁶
Dead cell conc. (cells/ml): 3.16 x 10⁴
Viability (%): 97.7
Average cell size (μm): 17.2

STO： Mouse embryonic fibroblast

Protocol

Results

Noise reduction: 5
Roundness: 0%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium

Total cell conc. (cells/ml): 1.78 x 10⁶
Live cell conc. (cells/ml): 1.50 x 10⁶
Dead cell conc. (cells/ml): 2.81 x 10⁵
Viability (%): 84.2
Average cell size (μm): 13.1

U-2 OS： Human osteosarcoma

Protocol

Results

Noise reduction: 5
Roundness: 60%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium

Total cell conc. (cells/ml): 7.66 x 10⁵
Live cell conc. (cells/ml): 7.21 x 10⁵
Dead cell conc. (cells/ml): 4.45 x 10⁴
Viability (%): 94.2
Average cell size (μm): 16.0

U937： Human lymphoma

Protocol

Results

Noise reduction: 5
Roundness: 40%
Min. cell size (μm): 3
Max. cell size (μm): 60
Declustering level: Medium

Total cell conc. (cells/ml): 1.22 x 10⁶
Live cell conc. (cells/ml): 1.12 x 10⁶
Dead cell conc. (cells/ml): 1.02 x 10⁵
Viability (%): 91.6
Average cell size (μm): 13.4

*Although it became one of the most important cell lines in medical research, it’s imperative that we recognize Henrietta Lacks’ contribution to science happened without her consent. This injustice, while leading to key discoveries in immunology, infectious disease, and cancer, also raised important conversations about privacy, ethics, and consent in medicine.
To learn more about the life of Henrietta Lacks and her contribution to modern medicine, click here.
http://henriettalacksfoundation.org/

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