NIR Solutions for Multiplexed Confocal Imaging

Interested in imaging with far-red fluorescent probes? Expand your multiplex capabilities with near-infrared (NIR) modules for the FV3000 microscope, including a 730 nm or 785 nm diode laser and GaAs PMTs for high-sensitivity detection up to 890 nm.

Greater multiplexing capabilities
Reduced autofluorescence
Gentler live-cell imaging
Deeper imaging with less absorption and scattering

Learn more about NIR and multiplexing

NIR Laser Diodes

Less cross-talk when selecting dyes
Laser diodes (LD730/785) designed to be driven in continuous wave (CW) mode, offering stability, low maintenance, and a long lifetime

Applicable dyes for NIR imaging

Laser	Fluorescent dye	λ_Ex (nm)	λ_Em (nm)
LD730	ATTO 725	727	752
	Cy7	743	767
	ATTO 740	743	763
	DiR	748	780
	DyLight 750	751	772
	Alexa Fluor 750	752	779
LD785	IRDye 800RS	767	785
	DyLight 800	770	795
	IRDye 800CW	778	794
	Alexa Fluor 790	782	805
	Cy7.5	790	810

	Cooled GaAs PMT Detector for NIR Imaging Significantly extends the detection range in the near-infrared High sensitivity up to 890 nm One or two GaAs PMT detectors can be combined with GaAsP or standard PMT detectors, enabling up to six individual detectors for multiplex imaging from the VIS to NIR Efficient cooling reduces thermal noise emitted from the photocathode for a high signal-to-noise ratio at extremely low light levels

Cooled GaAs PMT Detector for NIR Imaging

Significantly extends the detection range in the near-infrared
High sensitivity up to 890 nm
One or two GaAs PMT detectors can be combined with GaAsP or standard PMT detectors, enabling up to six individual detectors for multiplex imaging from the VIS to NIR
Efficient cooling reduces thermal noise emitted from the photocathode for a high signal-to-noise ratio at extremely low light levels

High Transmittance Optics for NIR Imaging

High transmittance from VIS to IR with silver-coated scan mirrors and Olympus’ unique 1600 Coating on the scan lens.

Deep Tissue Observation with Silicone Oil Immersion Objectives in the NIR

NIR fluorescence works well for deep imaging because NIR light scatters less in live tissue
The refractive index of silicone oil (ne≈1.40) is close to that of living tissue (ne≈1.38), enabling high-resolution observations deep inside living tissue with minimal spherical aberration
The UPLSAPO30XIR (NA 1.05, WD 800 μm) offers higher NIR transmittance thanks to Olympus’ 1600 Coating

Multiplexing with X Line High-Performance Objectives

Greater multiplexing imaging is possible using additional NIR detection channels
Reduced chromatic aberration during co-localization analysis (corrected at 400 nm–1000 nm)
Higher numerical aperture, excellent image flatness, and high transmittance from UV to NIR

TruFocus Red Z-Drift Compensator

Uses a near-infrared laser to locate the coverslip interface and corresponding focal position
Near-infrared light minimizes cell damage during prolonged observation
Works well for live cell imaging with far-red and near-infrared fluorophores

On-Demand Webinar

This webinar will focus on fluorescence multiplexing and deep tissue imaging using near infrared (NIR) laser light. NIR laser sources can help in visualizing biological structures more clearly and at higher resolution deep within the specimen. NIR excitation can also enable the use of more fluorescent dyes without spectral overlap.

Watch this webinar

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