MAP2 (green) and Hoechst (blue) in a cortical organoid at DIV 45 with cell line KOLF2.1J. Sample courtesy of: Declan J. Brennan, Nygaard Lab at UBC.

Absolute Quantitation—Every Pixel, Every Run

The new benchmark in advanced microscopy, our SilVIR™ detectors deliver photon-level quantitation with exceptional sensitivity and ultra-high signal-to-noise across the industry’s widest dynamic range. Trust the industry’s first built-in laser power monitor to ensure consistent, reproducible illumination from samples captured today and in the future.

Finally, combine beautiful images with beautifully quantifiable results.

SilVIR™ Next-Generation Detector Technology

Built on Evident’s patented silicon photomultiplier design, SilVIR technology captures every photon with exceptional sensitivity and the industry’s widest dynamic range. Low-noise electronics and a built-in laser power monitor maintain illumination stability and ensure reproducible, quantitative results from faint signals to deep-tissue imaging.

Expect exceptional imaging from every level of signal. Clear high and ultra-low signals from the same sample with no compromise in quality. Histograms show discrete photon counts with quantifiable intensity and minimum background.

Two Scanners, One Workflow: High-Density Sampling and High-Speed Imaging. No Compromises.

Whether you need high-speed series or pixel-dense maps, the FV5000 adapts. Our resonant scanner captures fast cellular dynamics at full clarity across a 20 mm FOV with little to no averaging, giving you high-quality, high-SNR raw images, in up to 438 FPS.

Switch to 8K × 8K galvo scanning to image large areas at high spatial resolution with ultra-fast pixel dwell times as short as 0.2 µs. Achieve up to 120 nm XY resolution across six spectral channels with high NA objectives and FV-OSR software—no additional hardware required.

The result: fewer compromises and faster time to data.

Stitched mouse brain slice cleared with SeeDB2. EYFP is expressed in cortical layer 5 pyramidal neurons in Thy1-YFP-H transgenic mice, acquired with a LUPLAPO25XO objective lens and resonant scanner. Sample courtesy of: Drs. Satoshi Fujimoto and Takeshi Imai, Graduate School of Medical Sciences, Kyushu University.

Effortless Super-Resolution Imaging

Powerfully Accessible Subcellular Answers

Achieve super-resolution imaging on the FV5000 with no additional hardware. By pairing high NA objectives, such as our A Line™ HR series, with FV-OSR software, you can resolve subcellular structures down to 120 nm in XY with ease.

FV-OSR automatically adjusts the confocal aperture to capture and enhance high-frequency signal components, producing crisp, detailed images in real time. Combined with the sensitivity of the SilVIR detector, the FV5000 delivers simultaneous super resolution across up to six spectral channels.

Take your imaging—and your discoveries—further than ever before.

Cultured HeLa cells expressing Lifeact-mScarlet-I and EB3-3xmNeonGreen. Acquired with super-resolution mode on the FV5000. Sample courtesy of: Haruka Mii, Prof. Kazuhiro Aoki, Graduate School of Biostudies, Kyoto University. To learn more about the life of Henrietta Lacks and her contribution to modern medicine, visit henriettalacksfoundation.org.

Capture Fast Responses

Thanks to the high SNR of the SilVIR detector, only minimal image averaging or accumulation is required to achieve high-quality results.

Live imaging of an acute olfactory bulb slice expressing GCaMP6f cleared with SeeDB-Live. Image acquired with a LUPLAPO25XS objective lens at 70 µm depth from the surface. Sample courtesy of: Drs. Shigenori Inagaki, Takeshi Imai, Graduate School of Medical Sciences, Kyushu University.

Live imaging of an acute olfactory bulb slice expressing GCaMP6f cleared with SeeDB-Live. Image acquired with a LUPLAPO25XS objective lens at 120 µm depth from the surface. Sample courtesy of: Drs. Shigenori Inagaki, Takeshi Imai, Graduate School of Medical Sciences, Kyushu University.

Live imaging of an acute olfactory bulb slice expressing GCaMP6f cleared with SeeDB-Live. Image acquired with a LUPLAPO25XS objective lens at 200 µm depth from the surface. Sample courtesy of: Drs. Shigenori Inagaki, Takeshi Imai, Graduate School of Medical Sciences, Kyushu University.

“The quality of imaging, I’ve never seen something similar before."

Soraya Villaseca, PhD
Department of Physiology, Development and Neuroscience, University of Cambridge

End-to-End Simplicity with FLUOVIEW Smart™ Software

Inspired by real researchers tackling the challenges of science, the FV5000 is transforming confocal imaging, making it smarter and faster.

• Smart Sample Search quickly locates your sample in XY and Z
• Smart Auto Laser Power Adjustment uses AI to optimize signal-to-noise across power setting
• Intuitive interface captures complex multidimensional images in just a few clicks
• Intelligent Shading Correction automatically creates seamless, high-quality stitched images

Note: FLUOVIEW Smart is available with FV5000 inverted configurations. FLUOVIEW Smart is not compatible with gantry, upright, or MPE configurations.

Read more about how Evident is revolutionizing confocal microscopy with FLUOVIEW Smart’s intuitive AI-enhanced workflows.

Intelligent Shading Correction

For edge-to-edge clarity and precision, automatically create seamless, high-quality stitched images with Intelligent Shading Correction.

Left: Without auto correction collar. Right: With auto correction collar. Automated correction collar adjustment delivers sharper, more detailed images.

Left: Without Intelligent Shading Correction. Right: With Intelligent Shading Correction. Intelligent Shading Correction automatically compensates for uneven illumination across the field of view to generate stitched images without manual adjustment, improving efficiency and consistency in large-area imaging.

Image Segmentation Powered by Deep Learning

Go Beyond Conventional Thresholds

Traditional intensity-based thresholding can be slow, inconsistent, and highly sensitive to sample conditions.

TruAI image segmentation uses deep learning to recognize subtle patterns and faint signals that conventional methods miss, enabling accurate, reproducible segmentation of weakly labeled structures and complex tissues.

Clearly distinguish cells—and quickly find answers.

Spheroid imaging and analysis with TruAI. Left: Without TruAI. Right: With TruAI. TruAI segments and classifies cells (images on the right), even at high-penetration depths when the nuclei DAPI signal becomes weaker due to scattering.

Engineered for Long-Term Precision, Built to Last

The FV5000 is engineered for long-term precision and adaptability, delivering the reliability and flexibility your science demands. Configure your system for today’s needs and expand effortlessly as your research evolves.

Add detectors, cameras, or lasers as workflows advance, or upgrade to multiphoton imaging with the MPE module, enabling single- and multiphoton acquisition as well as second and third harmonic generation. Smart hardware and software continuously monitor and optimize imaging performance, ensuring consistent, reproducible results. And with Evident’s global service and support network, every system is built to deliver lasting confidence and uptime.

Built for Reproducibility

The FV5000 maintains measurement precision through active system monitoring. The Laser Power Monitor (LPM) ensures consistent laser output across sessions, allowing different users to acquire images under identical conditions, even days or weeks apart. This stability supports the reproducibility required for quantitative and longitudinal studies.

To further safeguard performance, the Microscope Performance Monitor (MPM) automatically evaluates system sensitivity and imaging consistency. It detects deviations early, helping researchers maintain confidence in their results and ensuring every dataset reflects true experimental conditions.

Read the White Paper

Configurations

Designed for Every Application

The FV5000 platform supports a full range of configuration, from IX85 inverted systems for high-speed live-cell imaging and upright frames for general imaging or electrophysiology, to gantry setups for large or irregular specimens. For deeper imaging, the MPE (multiphoton excitation) configuration enables small-animal and thick-tissue studies, including large-frame and 3D organoid-optimized designs.

Confocal and multiphoton modes can also be combined in a single system, giving researchers unmatched versatility within one platform. Up to six SilVIR detector channels can be configured for confocal imaging and an additional six for multiphoton detection, offering up to 12 simultaneous channels with photon-level sensitivity across modalities.

Upright System

For glass slide sample imaging.

Upright System

For electrophysiology.

Gantry System

For in vivo observation that requires maximum space.

Inverted System

For observing tissue cultures, 3D cultures, and cell cultures (spheroids).

Upright System

A large focus stroke accommodates a range of specimens, from tissue slices to live mice and other small animals.

Gantry System

The frame maintains a large working space below the objective, making it easier to position experiment equipment.

Inverted System

The frame supports observation of 3D cultures and multicellular clusters that are difficult to image using an upright frame.

Learn more about the FV5000MPE for multiphoton applications.

Driving the Future of High-End Imaging Research

The FV5000 combines precision engineering with intelligent automation to deliver reliable, reproducible results for every user—from core facilities to individual researchers.

• Core Facilities: Long-term stability with SilVIR detectors, automated calibration, and a modular, future-ready design that minimizes downtime and simplifies management.
• Research Leaders: Quantitative, publication-quality data through photon-level detection and automated workflows that accelerate discovery.
• Individual Users: Intuitive operation, rapid setup, and integrated performance monitoring ensure confident imaging from day one.

Support and Service You Can Count On

When it comes to protecting your investment and the integrity of your research, your needs come first. We stand behind our products with a commitment to prompt service and technical support to help you achieve your goals.

Available in three convenient tiers—Maintenance, Protection, and Performance Plus—our FV5000 Service Plans* include priority support to help minimize downtime, regular scheduled maintenance to keep your equipment in peak condition, predictable repair costs to eliminate unplanned expenses, and direct, efficient solutions when you need them most.

^{*Regional variations in service offerings may apply.}