Overview

	Olympus UIS2 fluorescence mirror units are optimized to maximize fluorophore performance, producing uniform and highly efficient light transmittance. The filter efficiency enables the use of weaker excitation light to minimize photobleaching and sample damage. Get in Touch

Olympus UIS2 fluorescence mirror units are optimized to maximize fluorophore performance, producing uniform and highly efficient light transmittance. The filter efficiency enables the use of weaker excitation light to minimize photobleaching and sample damage.

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Filter Cubes for BX3, IX3 Microscopes, and APX100 system

Download the transmittance wavelength data for U-F Series

	Unit Name	Excitation Filter	Emission Filter	Dichromatic Mirror
Ultraviolet Excitations	Loading gallery... Loading... U-FUW	340-390	420IF	410
	Loading gallery... Loading... U-FUN	360-370	420IF	410
	Loading gallery... Loading... U-FUNA	360-370	420-460	410
Blue Violet Excitations	Loading gallery... Loading... U-FBVW	400-440	460IF	455
Blue Excitations	Loading gallery... Loading... U-FBW	460-495	510IF	505
	Loading gallery... Loading... U-FBWA	460-495	510-550	505
	Loading gallery... Loading... U-FBN	470-495	510IF	505
	Loading gallery... Loading... U-FBNA	470-495	510-550	505
Green Excitations	Loading gallery... Loading... U-FGW	530-550	575IF	570
	Loading gallery... Loading... U-FGWA	530-550	575-625	570
	Loading gallery... Loading... U-FGNA	540-550	575-625	570
Yellow Excitations	Loading gallery... Loading... U-FYW	540-585	600IF	595
Fluorescent Protein Variants	Loading gallery... Loading... U-FCFP	425-445CFP	460-510CFP	455CFP
	Loading gallery... Loading... U-FGFP	460-480GFP	495-540GFP	490GFP
	Loading gallery... Loading... U-FYFP	490-500YFP	515-560YFP	515YFP
	Loading gallery... Loading... U-FRFP	535-555HQ	570-625HQ	565HQ
	Loading gallery... Loading... U-FMCHE	565-585	600-690	595

Filter Cubes for IX3 Microscopes

	Unit Name	Excitation Filter	Emission Filter	Dichromatic Mirror
Ultraviolet Excitations	Loading gallery... Loading... IX3-FUWXL	340-390	420IF	410
Blue Violet Excitations	Loading gallery... Loading... IX3-FBVWXL	400-440	460IF	455
Green Excitations	Loading gallery... Loading... IX3-FGWXL	530-550	575IF	570
Fluorescent Protein Variants	Loading gallery... Loading... IX3-FCFPXL	425-445CFP	460-510CFP	455CFP
	Loading gallery... Loading... IX3-FGFPXL	460-480GFP	495-540GFP	490GFP
	Loading gallery... Loading... IX3-FYFPXL	490-500YFP	515-560YFP	515YFP
	Loading gallery... Loading... IX3-FMCHEXL	565-585	600-690	595

Filter Cubes for IX2, BX2, and MVX10 Microscopes

Download the transmittance wavelength data for U-M Series

	Unit Name	Excitation Filter	Emission Filter	Dichromatic Mirror
Ultraviolet Excitations	Loading gallery... Loading... U-MWU2	330-385	420	400
	Loading gallery... Loading... U-MNU2	360-370	420	400
	Loading gallery... Loading... U-MNUA2	360-370	420-460	400
Violet Excitations	Loading gallery... Loading... U-MNV2	400-410	455	455
Blue Violet Excitations	Loading gallery... Loading... U-MWBV2	400-440	475	455
Blue Violet Excitations	Loading gallery... Loading... U-MNBV2	420-440	475	455
Blue Excitations	Loading gallery... Loading... U-MSWB2	420-480	520IF	500
	Loading gallery... Loading... U-MWB2	460-490	520IF	500
	Loading gallery... Loading... U-MNB2	470-490	520IF	500
	Loading gallery... Loading... U-MWIB3	460-495	510IF	505
	Loading gallery... Loading... U-MWIBA3	460-495	510-550	505
	Loading gallery... Loading... U-MNIB3	470-495	510IF	505
	Loading gallery... Loading... U-MNIBA3	470-495	510-550	505
Green Excitations	Loading gallery... Loading... U-MWG2	510-550	590	570
	Loading gallery... Loading... U-MNG2	530-550	590	570
	Loading gallery... Loading... U-MWIG3	530-550	575IF	570
	Loading gallery... Loading... U-MWIGA3	530-550	575-625	570
	Loading gallery... Loading... U-MNIGA3	540-550	575-625	570
Yellow Excitations	Loading gallery... Loading... U-MWIY2	545-580	610IF	600
Fluorescent Protein Variants	Loading gallery... Loading... U-MCFPHQ	425-445HQ	460-510HQ	450
	Loading gallery... Loading... U-MGFPHQ	460-480HQ	495-540HQ	485
	Loading gallery... Loading... U-MYFPHQ	490-500HQ	515-560HQ	505
	Loading gallery... Loading... U-MRFPHQ	535-555HQ	570-625HQ	565

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Selection Chart

Fluorescent Protein Variants

	Excitation Maximum (nm)	Emission Maximum (nm)	Applicable BX3/IX3/APX100 Mirror Unit	Applicable BX2WI/MVX10 Mirror Unit
AmCyan	458	489	U-FCFP, U-FBVW	U-MWBV2, U-MNBV2, U-MCFPHQ
Azami-Green	492	505	U-FBWA, U-FBNA, U-FGFP, U-FBW, U-FBN	U-MWIB3, U-MWIBA3, U-MNIB3, U-MNIBA3, U-MGFPHQ
DsRed2	563	582	U-FGW, U-FGWA, U-FGNA, U-FRFP	U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ
EBFP	380	440	U-FUNA, U-FUW, U-FUN	U-MWU2, U-MNU2, U-MNUA2, U-MNV2
ECFP	433	475	U-FCFP, U-FBVW	U-MCFPHQ, U-MWBV2, U-MNBV2, U-MNV2
EGFP	488	507	U-FBWA, U-FBNA, U-FGFP, U-FBW, U-FBN	U-MGFPHQ, U-MNIB3, U-MWIBA3, U-MWIB3, U-MNIBA3
EYFP	513	527	U-FBWA, U-FBNA, U-FYFP, U-FBW, U-FBN	U-MYFPHQ, U-MWIBA3, U-MNIBA3, U-MWIBA3, U-MNIBA3, U-MNIB3, U-MNB2, U-MWB2, U-MWIB3
Fucci (mAG1)	492/551	505/565	U-FBNA, U-FGFP, U-FBWA, U-FBW, U-FBN	U-MNIBA3, U-MGFPHQ, U-MNIB3, U-MWIBA3, U-MWIB3
GFP (S65T)	488	510	U-FBWA, U-FBNA, U-FGFP, U-FBW, U-FBN	U-MGFPHQ, U-MWIBA3, U-MNIBA3, U-MWIB3, U-MNIB3
GFP (WT)	395, 475	510	U-FBWA, U-FBNA, U-FGFP, U-FBW, U-FBN	U-MWIBA3, U-MNIBA3, U-MGFPHQ, U-MWIB3, U-MNIB
HcRed	588	618	U-FYW	U-MWIY2
Midoriishi-Cyan	472	495	U-FBWA, U-FBW, U-FBNA, U-FBN, U-FCFP, U-FGFP	U-MWIBA3, U-MWIB3, U-MNIBA3, U-MNIB3, U-MCFPHQ, U-MGFPHQ
mCherry	580	610	U-FMCHE, U-FYW	U-MWIY2
mRFP	584	607	U-FMCHE, U-FYW	U-MWIY2
mStrawberry	574	596	U-FMCHE, U-FYW	U-MWIY2
RFP (DsRed)	558	583	U-FRFP, U-FGW, U-FGWA, U-FGNA	U-MRFPHQ, U-MWIG3, U-MWIGA3, U-MNIGA3
Venus	515	528	U-FYFP	U-MYFPHQ, U-MNB2, U-MNIB3, U-MNIBA3, U-MSWB2, U-MWB2, U-MWIB3, U-MWIBA3

Photoactivation

	Excitation Maximum (nm)	Emission Maximum (nm)	Applicable BX3/IX3/APX100 Mirror Unit	Applicable BX2WI/MVX10 Mirror Unit
Dronpa-Green	503	518	U-FBN, U-FBNA, U-FBW, U-FBWA, U-FYFP	U-MGFPHQ, U-MNB2, U-MNIB3, U-MNIBA3, U-MWB2, U-MWIB3, U-MWIBA3, U-MYFPHQ
PA-GFP	504	517	U-FGFP, U-FBWA, U-FBNA, U-FBW, U-FBN	U-MGFPHQ, U-MNIB3, U-MWIBA3, U-MWIB3, U-MNIBA3

Photoconversion

	Excitation Maximum (nm)	Emission Maximum (nm)	Applicable BX3/IX3/APX100 Mirror Unit	Applicable BX2WI/MVX10 Mirror Unit
Kaede (Green/Red)	509/573	518/582	U-FBN, U-FBNA, U-FBW, U-FBWA, U-FGFP, U-FYFP, U-FGW, U-FGWA, U-FGNA, U-FRFP	U-MWIB3, U-MWIBA3, U-MGFPHQ, U-MYFPHQ, U-MNB2, U-MNIB3, U-MNIBA3,U-MWB2, U-MNIGA3, U-MRFPHQ, U-MWIG3, U-MWIGA3

Carbohydrate Modification, Protein Modification (Labeling)

Protein modification

	Excitation Maximum (nm)	Emission Maximum (nm)	Applicable BX3/IX3/APX100 Mirror Unit	Applicable BX2WI/MVX10 Mirror Unit
Alexa Fluor 350	346	442	U-FUNA, U-FUW, U-FUN	U-MNUA2, U-MWU2, U-MNU2
Alexa Fluor 405	402	421	U-FUNA, U-FUW, U-FUN	U-MNUA2, U-MWU2, U-MNU2
Alexa Fluor 430	434	539	U-FBVW	U-MWBV2, U-MNBV2, U-MNV2
Alexa Fluor 488	495	519	U-FBWA, U-FBNA, U-FBW, U-FBN	U-MWIBA3, U-MNIBA3, U-MWIB3, U-MNIB3,UMNB2, U-MWB2
Alexa Fluor 532	531	554	U-FGW, U-FGWA, U-FGNA, U-FRFP	U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ
Alexa Fluor 546	556	573	U-FGW, U-FGWA, U-FGNA, U-FRFP	U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ
Alexa Fluor 555	555	567	U-FGW, U-FGWA, U-FGNA, U-FRFP	U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ
Alexa Fluor 568	578	603	U-FGW, U-FGWA, U-FGNA, U-FRFP, U-FYW, U-MCHE	U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ, U-MWIY2
Alexa Fluor 594	590	617	U-FYW, U-FMCHE	U-MWIY2
Allophycocyanin (APC)	650	660	U-FYW	U-MWIY2
7-amino-4-methylcoumarin-3-acetic acid (AMCA)	349	448	U-FUNA, U-FUW, U-FUN	U-MNUA2, U-MWU2, U-MNU2
BODIPY FL	505	513	U-FBWA, U-FBW, U-FBNA, U-FBN, U-FGFP	U-MWIBA3, U-MWIB3, U-MNIBA3, U-MNIB3
Cascade Blue	376	425	U-FUNA, U-FUW, U-FUN	U-MNUA2, U-MWU2, U-MNU2
Fluorescein-isothiocyanate (FITC)	494	518	U-FBWA, U-FBW, U-FBNA, U-FBN, U-FGFP, U-FYFP	U-MWIBA3, U-MNIBA3, U-MWB2, U-MNB2, U-MWIB3, U-MNIB3, U-MGFPHQ, U-MYFPHQ
Oregon Green 488	496	524	U-FBWA, U-FBW, U-FBNA, U-FBN, U-FGFP, U-FYFP	U-MWIBA3, U-MNIBA3, U-MWB2, U-MNB2, U-MWIB3, U-MNIB3, U-MGFPHQ, U-MYFPHQ
Phycoerythrin B (PE-B)	545	576	U-FGW, U-FGWA, U-FGNA, U-FRFP	U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ
Phycoerythrin R (PE-R)	490, 565	578	U-FGNA, U-FGW, U-FGWA, U-FRFP	U-MNIGA3, U-MWIG3, U-MWIGA3, U-MRFPHQ
Rhodamine B-isothiocyanate (RITC)	543	580	U-FGW, U-FGWA, U-FGNA, U-FRFP	U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ
Texas Red	595	615	U-FYW, U-MCHE	U-MWIY2
Tetramethylrhodamine-isothyocyanate (TRITC)	541	572	U-FGW, U-FGWA, U-FGNA, U-FRFP	U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ
1-Anilinonaphthalene-8-sulfonic acid (ANS)	365	460	U-FUW, U-FUN	U-MWU2, U-MNU2
CPM (7-Diethylamino-3-(4'-Maleimidylphenyl)-4-Methylcoumarin)	~384	470	U-FUW, U-FUN	U-MWU2, U-MNU2
Cy2	489	506	U-FBWA, U-FBNA, U-FBW, U-FBN	U-MWIBA3, U-MNIBA3, U-MWIB3, U-MNIB3
Cy3	(512); 550	570; (615)	U-FGW, U-FGWA, U-FGNA	U-MWIG3, U-MWIGA3, U-MNIGA3
Cy3.5	581	594; (640)	U-FYW, U-FMCHE	U-MWIY2
Dans　(1-Dimethyl Amino Naphaline 5 Sulphonic Acid)	340	525	U-FUW, U-FUN	U-MWU2, U-MNU2
Dansyl chloraide	360	520	U-FUW, U-FUN	U-MWU2, U-MNU2
Dansyl hydrazine	340	525	U-FUW, U-FUN	U-MWU2, U-MNU2
Eosin	525	545	U-FBWA, U-FBNA, U-FBW, U-FBN	U-MWIBA3, U-MNIBA3, U-MWIB3, U-MNIB3
Fluorescamine	390	465/475	U-FUW, U-FUN, U-FNUA	U-MWU2, U-MNU2, U-MNUA2, U-MNV2
FluorX	494	520	U-FBWA, U-FBNA, U-FBW, U-FBN	U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MSWB2, U-MWB2, U-MNB2
Lucifer Yellow CH	425	528	U-FBVW, U-FBW, U-FBW, U-FBWA	U-MWBV2, U-MNBV2, U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MWB2, U-MNIB2, U-MSWB2
Monochlorobimane (mBCI)	394	490	U-FUW, U-FUN, U-FUNA	U-MNUA2, U-MWU2, U-MNU2, U-MNV2
o-phtalaldehyde (OPT)	340	455	U-FUNA, U-FUW, U-FUN	U-MNUA2, U-MWU2, U-MNU2
Resorufin	572	583	U-FGW, U-FGWA, U-FGNA	U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWG2, U-MNG2
SITS (Primuline)	395-425	450	U-FUW, U-FUN, U-FUNA	U-MNUA2, U-MWU2, U-MNU2, U-MNV2
SITS (Stilbene Isothiosulphonic acid)	365	460	U-FUW, U-FUN, U-FUNA	U-MNUA2, U-MWU2, U-MNU2

Carbohydrate modification

	Excitation Maximum (nm)	Emission Maximum (nm)	Applicable BX3/IX3/APX100 Mirror Unit	Applicable BX2WI/MVX10 Mirror Unit
Acrifl avine	436	520	U-FBVW, U-FBW	U-MWBV2, U-MNBV2, U-MWB2, U-MSWB2

Nuclei, Nucleic Acid Staining

Nuclei, nucleic acid

	Excitation Maximum (nm)	Emission Maximum (nm)	Applicable BX3/IX3/APX100 Mirror Unit	Applicable BX2WI/MVX10 Mirror Unit
Acridine Orange	500	530/630-650	U-FBW, U-FBN, U-FBWA, U-FBNA, U-FYFP	U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
Acridine Yellow	470	550	U-FBW, U-FBN, U-FBWA, U-FBNA	U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MWB2, U-MNIB2, U-MSWB2
Acrifl avine-Feulgen	455	515	U-FBVW, U-FBW, U-FBWA	U-MWBV2, U-MNBV2, U-MWIB3, U-MWIBA3, U-MWB2, U-MSWB2
Auramine O-Feulgen	460	550	U-FBW, U-FBWA, U-FBNA	U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MWB2, U-MNIB2, U-MSWB2
7-Amino Actinomycin D	546	647	U-FYW, U-FGW, U-FMCHE	U-MWIG3, U-MWG2, U-MNG2, U-MWIY2
bis-Aminophenyl-oxadizole (BAO) -Feulgen	380	470	U-FUW, U-FUN, U-FUNA	U-MWU2, U-MNU2, U-MNUA2
BOBO 1	462	481	U-FBVW	U-MWBV2, U-MNBV2, U-MCFPHQ
BO PRO 1	462	481	U-FBVW	U-MWBV2, U-MNBV2
Chromomycin A3	445	575	U-FBVW	U-MWBV2, U-MNBV2, U-MSWB2
4, 6-diamidino-2-phenyl-indole HCI (DAPI)	345	455	U-FUW, U-FUN, U-FUNA	U-MWU2, U-MNU2, U-MNUA2
Ethidium Bromide	493	620	U-FGW, U-FGWA, U-FGNA	U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWG2, U-MNG2, U-SWG2
Hoechst 33258	345	478	U-FUW, U-FUN, U-FUNA	U-MWU2, U-MNU2, U-MNUA2
Hoechst 33342	343	483	U-FUW, U-FUN, U-FUNA	U-MWU2, U-MNU2, U-MNUA2
Mithramycin	445	575	U-FBVW	U-MWBV2, U-MNBV2, U-MSWB2
Olivomycin	430	545	U-FBVW	U-MWBV2, U-MNBV2
Pararosaniline-Feulgen	570	625	U-FYW, U-FMCHE, U-FGW, U-FGWA, U-FGNA	U-MWIY2, U-MWG2, U-MNG2, U-MWIG3, U-MWIGA3, U-MNIGA3
POPO 1	434	456	U-FBVW, U-FCFP	U-MNV2, U-MWBV2, U-MNBV2, U-MCFPHQ
Propidium Iodide (PI)	536	617	U-FGW, U-FGWA, U-FGNA, U-FYW	U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWIY2
Pyronin Y	540	570	U-FGW, U-FGWA, U-FGNA	U-MWIG3, U-MWIGA3, U-MNIGA3
Quinacrine Mustard	423	503	U-FBVW	U-MWBV2, U-MNBV2
SYTOX Green nucleic acid stain	504	523	U-FBW, U-FBN, U-FBWA, U-FBNA	U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
Thiazole Orange	510	530	U-FBW, U-FBN, U-FBWA, U-FBNA	U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
TOTO 1	509	533	U-FBW, U-FBN, U-FBWA, U-FBNA	U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
TOTO 3	642	661	U-FYW, U-FMCHE	U-MWIY2
TO PRO 3	642	661	U-FYW, U-FMCHE	U-MWIY2
YO PRO 1	491	509	U-FBW, U-FBN, U-FBWA, U-FBNA	U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
YOYO 1	491	509	U-FBW, U-FBN, U-FBWA, U-FBNA, U-FGFP, U-FYFP	U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3

Cell Viability Assessment

Living cell staining

	Excitation Maximum (nm)	Emission Maximum (nm)	Applicable BX3/IX3/APX100 Mirror Unit	Applicable BX2WI/MVX10 Mirror Unit
Acridine Orange	500	530/630-650	U-FBW, U-FBN, U-FBWA, U-FBNA, U-FYFP	U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
BCECF indicator	482(low pH) 503(high pH)	520(low pH) 528 (high pH)	U-FBW, U-FBN, U-FBWA, U-FBNA	U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
Calcein	494	517	U-FBW, U-FBN, U-FBWA, U-FBNA	U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
Carboxyfl uorescein diacetate (CFDA)	496	515	U-FBW, U-FBN, U-FBWA, U-FBNA	U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
Fluorescein diacetate (FDA)	496	515	U-FBW, U-FBN, U-FBWA, U-FBNA	U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3

Dead cell staining

	Excitation Maximum (nm)	Emission Maximum (nm)	Applicable BX3/IX3/APX100 Mirror Unit	Applicable BX2WI/MVX10 Mirror Unit
Ethidium Bromide	493	620	U-FGW, U-FGWA, U-FGNA	U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWG2, U-MNG2, U-SWG2
Ethidium homodimer-1	528	617	U-FGW, U-FGWA, U-FGNA, U-FYW	U-MWG2, U-MNG2, U-MWIG3, U-MWIGA3, U-MNIGA3
Propidium Iodide (PI)	536	617	U-FGW, U-FGWA, U-FGNA, U-FYW	U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWIY2

Membranes, Organelle, Membrane Potential

Membranes

	Excitation Maximum (nm)	Emission Maximum (nm)	Applicable BX3/IX3/APX100 Mirror Unit	Applicable BX2WI/MVX10 Mirror Unit
8-Anilino-1-naphthalene sulfonic acid (1,8-ANS)	385	485	U-FUW, U-FUN, U-FUNA	U-MWU2, U-MNU2, U-MNUA2
Dil	550	565	U-FGW, U-FGWA, U-FGNA	U-MWIG3, U-MWIGA3, U-MNIGA3
DiO	484	501	U-FBW, U-FBN, U-FBWA, U-FBNA	U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MSWB, U-MWB2, U-MNB2
Diphenylhexatriene (DPH)	351	430	U-FUW, U-FUN, U-FUNA	U-MWU2, U-MNU2, U-MNUA2
Filipin	350	480	U-FUW, U-FUN, U-FUNA	U-MWU2, U-MNU2, U-MNUA2
Fluorescamine	390	465	U-FUW, U-FUN	U-MWU2, U-MNU2, U-MNV2
Merocyanine 540	500	580	U-FGW	U-MWG2, U-MNG2, U-MWIG3

Organelle

	Excitation Maximum (nm)	Emission Maximum (nm)	Applicable BX3/IX3/APX100 Mirror Unit	Applicable BX2WI/MVX10 Mirror Unit
Acridine Orange	500	530/630-650	U-FBW, U-FBN, U-FBWA, U-FBNA, U-FYFP	U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
BODIPY FL Ceramide	503	512	U-FBW, U-FBN, U-FBWA, U-FBNA	U-MWIB3,U-MNIB3, U-MWIBA3, U-MNIBA3
DiO C6(3)	484	500	U-FBW, U-FBN, U-FBWA, U-FBNA	U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MSWB, U-MWB2, U-MNB2
ER Tracker Blue-White DPX	374	575	U-FUW, U-FUN	U-MWU2, U-MNU2, U-MNV2
JC-1	485	530	U-FBW, U-FBN, U-FBWA, U-FBNA	U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MSWB, U-MWB2, U-MNB2
Lyso Tracker Red DND-99	577	590	U-FGW, U-FGWA, U-FGNA, U-FYW	U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWIY2
Mito Tracker Green	490	516	U-FBW, U-FBN, U-FBWA, U-FBNA	U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
Mito Tracker Red CMXRos	579	599	U-FGW, U-FGWA, U-FGNA, U-FYW	U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWIY2
Nile Red (phospholipid)	552	636	U-FGW, U-FGWA, U-FGNA, U-FYW	U-MWG2, U-MNG2, U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWIY2
Rhodamine 123	507	530	U-FBW, U-FBN, U-FBWA, U-FBNA	U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
Pyronin Y	540	570	U-FGW, U-FGWA, U-FGNA	U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWG2, U-MNG2

Membrane potential

	Excitation Maximum (nm)	Emission Maximum (nm)	Applicable BX3/IX3/APX100 Mirror Unit	Applicable BX2WI/MVX10 Mirror Unit
Di-4-ANEPPS	497	710	U-FBW, U-FBN	U-MWIB3, U-MNIB3
Di-8-ANEPPS	465	635	U-FBW, U-FBN	U-MWIB3, U-MNIB3
FM 1-43	473	579	U-FBW, U-FBN	U-MWIB3, U-MNIB3
RH160	577	>610	U-FGW, U-FGWA, U-FGNA, U-FYW	U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWIY2
RH414	490	>662	U-FBW, U-FBN	U-MWIB3, U-MNIB3
RH421	541	>590	U-FGW, U-FGWA, U-FGNA	U-MWIG3, U-MWIGA3, U-MNIGA3
RH423	520-550	>610	U-FGW, U-FGWA, U-FGNA	U-MWIG3, U-MWIGA3, U-MNIGA3
RH795	560	650	U-FGW, U-FYW	U-MWIG3, U-MWIY2

Bacteria Staining, Bone Staining

Bacteria

	Excitation Maximum (nm)	Emission Maximum (nm)	Applicable BX3/IX3/APX100 Mirror Unit	Applicable BX2WI/MVX10 Mirror Unit
Acridine Orange	500	530/630-650	U-FBW, U-FBN, U-FBWA, U-FBNA, U-FYFP	U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
Acridine Yellow	470	550	U-FBW, U-FBN, U-FBWA, U-FBNA	U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MWB2, U-MNIB2, U-MSWB2
Auramine O	460	550	U-FBVW, U-FBW	U-MWBV2, U-MNBV2, U-MSWB2, U-MWB2, U-MWIB, U-MWIB3
Berberine sulfate	430	550	U-FBVW	U-MWBV2, U-MNBV2
Coriphosphine O	460	575	U-FBW	U-MWB2, U-MWIB3
CTC	430, 480	630	U-FBVW, U-FBW, U-FBN	U-MWBV2, U-MNBV2, U-MWIB3, U-MNIB3
Hoechst 33258	365	465	U-FUW, U-FUN, U-FUNA	U-MWU2, U-MNU2, U-MNUA2

Bone

	Excitation Maximum (nm)	Emission Maximum (nm)	Applicable BX3/IX3/APX100 Mirror Unit	Applicable BX2WI/MVX10 Mirror Unit
Acidic Fuchsin (Pararosaniline)	540	630	U-FGW, U-FGN	U-MWG2, U-MNG2, U-MWIG3
Calcein	495	520	U-FBW, U-FBN, U-FBWA, U-FBNA	U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
Calcein Blue	375	435	U-FUW, U-FUN, U-FUNA	U-MWU2, U-MNU2, U-MNUA2
Tetracycline	390	560	U-FUW, U-FBVW	U-MWU2, U-MNV2, U-MWBV2, U-MNBV2
Xylenol Orange	440, 570	610	U-FGW, U-FGN	U-MWG2, U-MNG2, U-MWIG3

Ion Concentrations

Calcium

	Excitation Maximum (nm)	Emission Maximum (nm)	Applicable BX3/IX3/APX100 Mirror Unit	Applicable BX2WI/MVX10 Mirror Unit
BTC	400/480	540	U-FBN, U-FBNA, U-FBW, U-FBWA	U-MNV, U-MNIB3, U-MNIBA3, U-MWIB3, U-MWIBA3
Calcium Green	505	532	U-FBN, U-FBNA, U-FBW, U-FBWA	U-MNIB3, U-MNIBA3, U-MWIB3, U-MWIBA3
Fluo 4	495	510	U-FBN, U-FBNA, U-FBW, U-FBWA	U-MNIB3, U-MNIBA3, U-MWIB3, U-MWIBA3
Oregon Green 488 BAPTA-1	494	523	U-FBN, U-FBNA, U-FBW, U-FBWA	U-MNIB3, U-MNIBA3, U-MWIB3, U-MWIBA3
Oregon Green 488 BAPTA-5N	494	521	U-FBN, U-FBNA, U-FBW, U-FBWA	U-MNIB3, U-MNIBA3, U-MWIB3, U-MWIBA3

pH

	Excitation Maximum (nm)	Emission Maximum (nm)	Applicable BX3/IX3/APX100 Mirror Unit	Applicable BX2WI/MVX10 Mirror Unit
BCECF	440/490	530	U-FBVW, U-FBN, U-FBNA, U-FBW, U-FBWA	U-MWBV, U-MNIB3, U-MNIBA3, U-MWIB3, U-MWIBA3

Nerve Cell Tracer, Others

Nerve cell tracer

	Excitation Maximum (nm)	Emission Maximum (nm)	Applicable BX3/IX3/APX100 Mirror Unit	Applicable BX2WI/MVX10 Mirror Unit
Evans Blue	470-540	680	U-FBW, U-FBN	U-MWIB3, U-MNIB3
Fast Blue	360	410	U-FUW, U-FUN, U-FUNA	U-MWU2, U-MNU2, U-MNUA2
Granular Blue	375	410	U-FUW, U-FUN, U-FUNA	U-MWU2, U-MNU2, U-MNUA2
True Blue	373	404	U-FUW, U-FUN, U-FUNA	U-MWU2, U-MNU2, U-MNUA2

Others

	Excitation Maximum (nm)	Emission Maximum (nm)	Applicable BX3/IX3/APX100 Mirror Unit	Applicable BX2WI/MVX10 Mirror Unit
Aniline blue	370	509	U-FUW, U-FUN	U-MWU2, U-MNU2
Alexa Fluor 488-Phalloidin	495	518	U-FBW, U-FBN, U-FBWA, U-FBNA	U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
Calcofl uor white	440	500-520	U-FUW, U-FUN	U-MWU2, U-MNU2, U-MNV2
Euchrysine (Acridine Orange)	500	530/630-650	U-FBW, U-FBN, U-FBWA, U-FBNA, U-FYFP	U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
NanoOrange	485	590	U-FBW, U-FBN	U-MWIB3, U-MNIB3
NBD-Phallacidin	465	536	U-FBW, U-FBN, U-FBWA, U-FBNA	U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
Rhodamine-Phalloidin	540	565	U-FGW, U-FGWA, U-FGNA	U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWG2, U-MNG2
sulforhodamine 101	586	605	U-FYW, U-FMCHE	U-MWIY2
Thiofl avin T	430	485	U-FBVW	U-MWBV2, U-MNBV2