Embryonic Rat Thoracic Aorta Medial Layer Myoblast Cells (A-10 Line) | Olympus LS

Home/ Learn/ Microscopy Resource Center/ Microscopy Primer/ Specialized Microscopy Techniques/ Fluorescence/ Digital Image Gallery - Fluorescence Microscopy/ Fluorescence Digital Image Gallery - Cells in Culture/ a10/ Embryonic Rat Thoracic Aorta Medial Layer Myoblast Cells (A-10 Line)

Embryonic Rat Thoracic Aorta Medial Layer Myoblast Cells (A-10 Line) — Embryonic Rat Thoracic Aorta Medial Layer Myoblast Cells (A-10 Line)
In order to label the intermediate filaments in a log phase adherent A-10 culture, the fixed and permeabilized cells were blocked and treated with mouse anti-vimentin (porcine eye lens) primary antibodies followed by goat anti-mouse secondary antibodies (IgG) conjugated to Texas Red-X. Filamentous actin was visualized with phalloidin conjugated to Oregon Green 488, while the nuclei were stained with Hoechst 33258.

Sorry, this page is not
available in your country.

Sorry, this page is not available in your country