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IXplore IX85 SpinSR Microscope

Overview
IXplore Spin

Stunning Detail, Faster Discovery: IXplore™ IX85 SpinSR Microscope

Capture ultra-small cellular dynamics in stunning detail with the IXplore™ IX85 SpinSR super-resolution microscope system. Designed for prolonged cell viability in time-lapse experiments, the IX85 SpinSR combines fast image acquisition, advanced algorithms, and XY resolution down to 120 nm.

Capture ultra-small cellular dynamics in stunning detail with the IXplore IX85 SpinSR super-resolution microscope system.

  • Discover more with high-speed imaging in resolution down to 120 nm
  • Designed for prolonged cell viability in long-term time-lapse experiments
  • Powered by Yokogawa spinning disk technology
  • Enhanced with the IXplore IX85 platform and Evident’s TruSight™ SR super-resolution algorithm
  • Learn how the TruSight™ SR algorithm achieves super-resolution imaging

µm maximum intensity projection of a mouse brain section, GFP. Scale bar 2 um. Sample courtesy of SunJin Lab Co., Taiwan.

Rapid Super Resolution for Live Cells

With fast image acquisition and advanced algorithms, the IXplore IX85 SpinSR makes capturing super-resolution details even easier so you can discover new insights faster. Observe dynamic subcellular processes with fast, gentle live-cell imaging.

Confocal image

Super-resolution image

NIH3T3 cells labeled with Hoechst (blue), β-tubulin–AF555 (green), CPCA–HSP60–AF647 (magenta), and fibrillarin–AF568 (gray). Scale bar: 5 µm. Sample courtesy of EnCor Biotechnology Inc.

Synchronized Imaging at High Speed

Featuring a fast frame rate for super-resolution imaging, the IXplore IX85 SpinSR rapidly acquires dynamic cellular events at the nanoscale with XY resolution down to 120 nm. Capture ultra-small cellular dynamics by imaging at speeds up to 200 fps at full frame.

Our real-time controller works as the system’s conductor, ensuring imaging is coordinated with microsecond accuracy. It delivers coordinated imaging even at the highest speeds, reducing the risk of sample damage with shorter exposures.

Live super-resolution imaging of LC3-EGFP expressing U2OS cells. Acquired with the UPLSAPO100XS objective lens.

Bright Super-Resolution Images of Live Cells

The high-sensitivity model equipped with a SoRa disk realizes brighter super resolution image acquisition thanks to the spinning disk with a microlens in the confocal pinhole. Each confocal pinhole enables you to image with lower laser power, reducing photobleaching and phototoxicity in your sample while enabling bright super-resolution images.

Image clarity depends on how the sample is illuminated and how efficiently the emitted light is collected. In a standard spinning disk confocal system, some light passes through the pinhole but may not be clearly resolved if it is off center. The SoRa disk addresses this by using microlenses to redirect each point of light toward the center of the pinhole, resulting in sharper, brighter images. This approach enhances resolution to nearly the best possible level—like what could be achieved with a theoretically infinitesimal pinhole.

Reference: T. Azuma and T. Kei, "Super-Resolution Spinning-Disk Confocal Microscopy Using Optical Photon Reassignment, " Opt. Express 23, 15003-15011 (2015).

Quantitative Super-Resolution Data

The TruSight SR algorithm preserves the original image’s intensity levels, enabling you to analyze and compare the brightness of samples in super-resolution images. These stable results support quantitative analysis.

Original image

With TruSight SR

Intensity histogram of original and TruSight SR images

Discover More on One Platform

As the needs of your facility grow, the IXplore IX85 SpinSR can be expanded to meet them. With flexible frame customization options, this super-resolution system adapts to a wide range of applications.

  • Supports a wide wavelength range from 405 to 640 nm, offering the flexibility to use the ideal wavelength for your fluorophores
  • Compatible with incubation systems and environmental controls for stable long-term experiments
  • Versatile imaging modes: Switch between widefield, confocal, and super-resolution imaging with one click

Seamless Workflows

The IXplore IX85 SpinSR is designed to drive efficiency with easy workflows for busy labs. Our cellSens™ software streamlines experiment setup and operation, providing you with more time to review results and make discoveries.

Optimized Performance, Trusted Results

Built on the stable IXplore IX85 platform and powered by Yokogawa spinning disk technology, the SpinSR system is optimized for high-performance super-resolution imaging. Our world-renowned optics add to this confidence, equipping your lab with a powerful set of tools to produce publication-ready images.

Your Sample in Focus

Capture high-precision, multipoint time-lapse images that are aligned and in focus with our TruFocus™ Z-drift compensator. With stable focus for long-term imaging and plate screening, you can be confident in the quality of your results.

LC3-EGFP expressing U2OS cells.

Range of Optimized Objectives

Evident’s expansive range of objectives can meet any research need, pushing your super-resolution system even further.

Simplified Immersion Imaging

The world’s first silicone gel pad objective (LUPLAPO25XS) combines all the quality and advantages of our silicone immersion objectives with the convenience and useability of a dry objective.

Left: confocal image

Our innovative multi-immersion objective with silicone gel pad technology (LUPLAPO25XS) combines all the quality and advantages of our silicone immersion objectives with the convenience and useability of a dry objective.

Right: TruSight SR image

XYZ image comparison between a LUPLAPO25XS silicone gel objective lens (left) and a UPLXAPO20X dry objective lens. Cleared HeLa* 1 cell spheroid (cyan: nuclei, magenta: microtubules). *To learn more about the origin of HeLa cells, visit henriettalacksfoundation.org.

Cultured Cos-7 cells, Tubulin Alexa Fluor 488. Easily switch from a confocal image to a higher resolution image using the LUPLAPO25XS objective lens with the TruSight SR super-resolution algorithm.

Consistent Quality

X Line series objectives redefine optical performance across applications. Taking full advantage of the IXplore IX85 SpinXL’s 26.5 mm FOV, X Line objectives deliver exceptional correction of spherical and chromatic aberrations throughout a wide field of view. With high numerical apertures (NA) and outstanding transmission, they provide sharp, consistent images.

Customized for Your Lab

Evident’s microscopy professionals will make sure you build the right IXplore IX85 SpinSR system for your lab’s unique needs. Customize every aspect of your spinning disk confocal super-resolution microscope, from the lasers to the eyepieces.

IXplore IX85 SpinSR (1-Deck System)

  • High-quality confocal imaging system with a wider FOV
  • Super-resolution imaging down to 120 nm resolution

IXplore IX85 SpinSR (2-Deck System)

  • High-quality confocal imaging system
  • Super-resolution imaging down to 120 nm resolution
  • 2-deck frame structure for expanding your experiments

IXplore Microscopes

IXplore™ IX85 SpinXL

See more and discover faster with an unparalleled 26.5 mm field of view (FOV).

  • Capture more fast cellular dynamics in a single frame at imaging speeds up to 498 fps Precise, gentle live-cell imaging maintains sample viability
  • Supports a wide range of applications and users with speed, precision, and flexibility
  • Streamlines tasks throughout the lab with intelligent automation and easy workflows
  • Evolves with your research via a wide wavelength range, tailored disk options, and expandable imaging modalities
  • Powered by CrestOptics spinning disk technology and enhanced with the IXplore™ IX85 platform
学习更多内容
 

IXplore™ IX85 SpinSR

Capture ultra-small cellular dynamics in stunning super-resolution detail.

  • Discover more with high-speed imaging in resolution down to 120 nm
  • Designed for prolonged cell viability in long-term time-lapse experiments
  • Powered by Yokogawa spinning disk technology Enhanced with the IXplore™ IX85 platform and Evident’s TruSight™ SR super-resolution algorithm
学习更多内容
 

IXplore™ IX85

IXplore IX85平台具有出色的可定制性,可助力您设计或打造出能满足自己特定目标的智能型、高性能成像系统。IXplore IX85拥有出色的26.5毫米视场数(FN),以及一系列先进的端到端成像和工作流程功能,这意味着您可以捕捉到比以往更多的细节,同时还大幅缩短了采集时间。

IXplore™ IX85 Live

IXplore™IX85 Live系统的设计旨在减少光漂白和光毒性,以达到优化针对活细胞和组织观察的生理实验。通过提供精准的环境控制和高稳定性,它能延长细胞存活时间并获得稳定的延时图像,适用用于如癌症、干细胞和大脑研究的应用。

  • 通过配合TruFocus™Z漂移补偿系统,在延时成像中能准确可靠地保持聚焦
  • 使用Olympus硅油物镜探索细胞的真实形态

IXplore™ IX85 Pro

IXplore IX85是IXplore IX85 Pro系统的基础,提供出色的视场角(FOV)和一系列先进的端到端成像功能,可使您看到和捕捉到比以往更多的细节,同时还大幅缩短了采集时间。使用IXplore IX85显微镜系统,体验非凡的速度、清晰度和可靠性。

IXplore™ IX85 Spin

IXplore™ IX85 Spin系统具有一个转盘共聚焦单元,可实现快速3D图像采集、宽广视场,并可在延时实验中延长细胞活性。研究人员可以使用它在更深的位置对更厚的样品执行具有高分辨率和对比度的快速3D共焦成像。转盘还有助于减少激发时样品的光漂白和光毒性。

  • TruFocus Z漂移补偿系统可保持每帧的聚焦效果
  • 使用X Line物镜进行改善了光收集效率的准确3D成像

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