Overview

Four-channel multiphoton dataset of cleared mouse intestine.

2K x 2K resonant volumetric imaging. High SNR with the SilVIR detector enables high-quality results with minimal averaging. Four-channel multiphoton dataset of cleared mouse intestine.

Deeper Discoveries and Advanced Multiphoton

The FV5000MPE enables quantitative imaging deep within thick, scattering samples, combining SilVIR detectors, TruSight™ deconvolution, and TruAI noise reduction for outstanding signal-to-noise and clarity. MPE-optimized objectives, TruResolution auto correction collar, and automated IR laser alignment maintain sharp focus throughout the imaging volume.

The compact fiber-pigtailed laser system offers an affordable, easily deployed solution for routine multiphoton imaging and rapid installation. For advanced applications, the fully tunable MPE laser configuration provides broad excitation flexibility and precise wavelength control for demanding experiments.

One-, two-, or three-line simultaneous MPE laser excitation delivers clarity and reproducibility millimeters deep.

From Compact to Advanced MPE Setups

Convenient Multiphoton Imaging

The FV5000MPE addresses some of the primary challenges associated with multiphoton excitation (MPE)—costly traditional pulsed tunable IR lasers and strict environmental conditions—with a new generation of compact single wavelength fiber-pigtailed IR lasers that are cost-effective, easy to handle, and designed to bring MPE within reach of a broader range of users.

Tunable Infrared Laser Solutions for Advanced Multiphoton Studies

For more advanced applications, the FV5000MPE supports the latest tunable infrared pulsed lasers for deep multiphoton imaging across 680–1300 nm. These IR lasers provide two laser lines—one tunable and one fixed at 1040/1045 nm—for efficient excitation of far-red fluorophores.

Human kidney organoid. Captured with single wavelength fiber-pigtailed IR lasers at 920 nm and 1064 nm for simultaneous 3CH multiphoton imaging with a LUPLAPO25XO objective lens.
Sample courtesy of: Dr Robert Turnbull and Prof. Katja Röper, Department of Physiology, Development and Neuroscience, University of Cambridge.

Axon FP lasers at 920 nm and 1064 nm installed in a 19-inch rack.

Single-wavelength fiber-pigtailed laser (Axon FP).

Tunable infrared pulsed lasers for deep multiphoton imaging.

Tunable infrared laser solutions for advanced multiphoton studies from SpectraPhysics and Coherent.

More Colors and More Information

Capture more colors and more information in one image with the FV5000MPE’s advanced multiplexing features. The FV5000MPE offers simultaneous multicolor imaging with up to six channels at once to capture more information in less time. Make the most of the SilVIR detector’s high sensitivity to quickly produce outstanding images at depth.

Learn More About SilVIR

Read the White Paper

Human iPSC-derived kidney organoids with membrane-GFP. Captured with single-wavelength fiber-pigtailed IR lasers at 920 nm and 1064 nm for simultaneous 3CH multiphoton imaging at 2K resonant imaging.
Sample courtesy of: Dr Robert Turnbull and Prof. Katja Röper, Department of Physiology, Development and Neuroscience, University of Cambridge.

Optimized Performance

Optimized Performance and Results

Auto Laser Alignment

The FV5000MPE’s four-axis auto laser alignment system simplifies maintenance by keeping the excitation beam precisely aligned within the scanner unit, even when wavelength tuning, temperature changes, or other factors cause drift. The system adjusts beam position and angle to maintain optimal laser power and consistent pixel registration.

In dual-laser configurations, auto alignment also preserves beam co-alignment, minimizing channel co-registration errors for accurate multi-line imaging. When needed, users can perform manual fine-tuning directly through the software interface.

Auto laser alignment for multiphoton imaging.

Left: Pixel shift. Right: Correction of pixel shift.

Comparison of a normal image and a Deep Focus image captured using the FLUOVIEW FV5000MPE multiphoton laser scanning microscope.

Left: Normal image. Right: Deep Focus mode image.

Maximize Signal with Deep Focus Mode

Deep Focus mode adjusts the laser beam diameter according to the sample’s scattering properties. In highly scattering in vivo specimens, the beam can be narrowed to allow more excitation photons to penetrate deeper into the tissue, resulting in bright high-resolution images even at greater depths.

TruResolution™ MPE Objectives

FV30-AC10SV Objective Lens
10X multi-immersion, NA 0.60, WD 8.0 mm

FV30-AC25W Objective Lens
25X water-immersion, NA 1.05, WD 2.0 mm

Top: With TruResolution. Bottom: Without TruResolution. XZ images of 0.2 μm fluorescent microspheres in scattering gel at various depths acquired using the FV30-AC25W objective lens.

Top: With TruResolution. Bottom: Without TruResolution. XZ images of 0.2 μm fluorescent microspheres in scattering gel (RI=1.36) at various depths acquired using the FV30-AC25W objective lens.

MPE Objectives

Objectives Designed for Depth

Sharper Imaging, Deeper Insights

Engineered for multiphoton excitation, A Line™ MPE objectives deliver high-precision imaging of biological specimens at depths of up to 8 mm, supporting both in vivo and transparent samples.

These objectives are optimized for performance and versatility, offering:

A diverse selection of models to match varied research needs
Optical designs combining a high numerical aperture, long working distance, and wide field of view
Compatibility with multiple immersion media and tissue-clearing agents
Built-in correction collars to compensate for refractive index mismatch and coverslip variations

Compare MPE Objectives

Wide fields of view enable these objectives to efficiently collect scattered fluorescence photons and generate brighter images from deep within specimens.

Dedicated Multiphoton Objective	NA	WD (mm)	Immersion Index
XLPLN10XSVMP	0.60	8.0	1.33–1.52
XLPLN25XWMP2	1.05	2.0	1.33
XLPLN25XSVMP2	1.00	4.0	1.33–1.40
XLSLPLN25XSVMP2	0.95	8.0	1.33–1.40
XLSLPLN25XGMP	1.00	8.0	1.41–1.52

Example of 1600 coating technology with the XLPLN25XWMP2 objective lens.

Optics with Outperforming IR Coating

Our innovative IR coating (1600 coating) for multiphoton objectives and scanner optics further refines deep observation quality.

Reliable Tissue Imaging

For upright microscope systems, our newly developed 16X multi-immersion objective (NA 0.8, WD 3.0 mm) offers an excellent balance between resolution and field of view—an ideal choice for broad tissue imaging applications. It has high transmittance from the VIS to NIR range, and enables seamless switching between multiphoton and confocal imaging.

XLPLFLN16XW / NA 0.80
Working Distance: 3.0 mm
Immersion Index: 1.33–1.52

Configurations

Built on the Versatile Platform

The FV5000MPE is part of the modular FV5000 family—engineered to cover every imaging need, from single-cell dynamics to deep-tissue and small-animal studies.

It can be configured as a dedicated multiphoton (MPE) system or as a combined confocal + multiphoton setup for seamless multimodal imaging.

Optimized designs support thick-tissue, organoid, and in vivo applications, while up to six SilVIR detector channels can be assigned to confocal and six to multiphoton detection—enabling up to 12 simultaneous SilVIR detector channels across modalities.

Learn more about the FV5000 confocal configuration here.

Multiple Frame Options

Depending on your application, you can choose between upright, gantry, or inverted microscope frames.

Upright Microscope System

A large focus stroke accommodates a range of specimens, from tissue slices to live mice and other small animals.

Gantry Microscope System

The frame maintains a large working space below the objective, making it easier to position experiment equipment.

Inverted Microscope System

The frame supports observation of 3D cultures and multicellular clusters that are difficult to image using an upright frame.

Six-channel non-descanned (NDD) module for the FLUOVIEW FV5000MPE

Six-channel non-descanned detection (NDD) module of the FLUOVIEW FV5000MPE. The modular SilVIR detector units enable simultaneous multicolor imaging and deep-tissue acquisition with exceptional sensitivity and unmatched dynamic range.

Create Your Own Custom Configuration

Take system customization to the next level by integrating your own optical components. For example, you can add a custom laser to an available port to enable advanced techniques such as three-photon or specialized imaging methods.

As your research evolves, your imaging system should evolve with it. The FV5000MPE platform supports specialized solutions* that extend the capabilities of standard configurations, helping you adapt to new applications and experimental requirements.

^{*Not available in some countries or regions.}

Support and Service You Can Count On

When it comes to protecting your investment and the integrity of your research, your needs come first. We stand behind our products with a commitment to prompt service and technical support to help you achieve your goals.

Available in three convenient tiers—Maintenance, Protection, and Performance Plus—our FV5000MPE Service Plans* include priority support to help minimize downtime, regular scheduled maintenance to keep your equipment in peak condition, predictable repair costs to eliminate unplanned expenses, and direct, efficient solutions when you need them most.

^{*Regional variations in service offerings may apply.}

Evident service and support for microscopy and imaging solutions

FV5000MPE Service Plans

	Maintenance	Protection	Performance Plus
Priority remote support	✓	✓	✓
Preventative maintenance	✓	✓	✓
Repair coverage (parts, labor, travel)	10% discount	✓	✓
Fast on-site response	-	-	✓

Specifications

FV5000MPE / FV5000MPE-RS Specifications

SPECIFICATIONS		FV5000MPE	FV5000MPE-RS
Scanner	Galvanometer Scanner	64 × 64 – 8192 × 8192 pixels, 0.2 μs/pixel – 1000 μs/pixel
	Resonant Scanner		512 × 512 pixels, 1024 × 1024 pixels, 2048 × 2048 pixels
	Field Number	20 (for both scanner types)
Spectral Confocal Detector	Detector	SilVIR detector (cooled SiPM, broadband type/red-shifted type)
	Maximum Channels	Six channels
	Spectral Method	VPH, detectable wavelength range 400-900 nm
Non-Descanned Detector	Detector	SilVIR detector (cooled SiPM, broadband type/red-shifted type)
Non-Descanned Detector	Maximum Channels	Six channels
CW Laser	VIS Laser	405 nm, 445 nm, 488 nm, 514 nm, 561 nm, 594 nm, 640 nm
CW Laser	NIR Laser	685 nm, 730 nm, 785 nm
IR Pulsed Laser	Tunable Laser	One-laser system, dual-laser-line system, two-laser system Excitation wavelength: 680-1300 nm Four-axis auto alignment, auto beam expander
IR Pulsed Laser	Single Wavelength Fiber Pigtailed Laser	920 nm, 1064 nm
Image	High dynamic range photon counting (1G cps, 16-bit)