Overview

	Excellent Multicolor TIRF Imaging Designed for membrane dynamics, single molecule detection, and colocalization experiments, the IXplore TIRF microscope system offers simultaneous multicolor TIRF imaging for up to 4 colors with high stability. Get in Touch

Excellent Multicolor TIRF Imaging

Designed for membrane dynamics, single molecule detection, and colocalization experiments, the IXplore TIRF microscope system offers simultaneous multicolor TIRF imaging for up to 4 colors with high stability.

Get in Touch

	Simultaneous Multicolor TIRF The Olympus cellTIRF system provides true simultaneous acquisition of up to four wavelengths. Motorized angle control for each laser provides equal evanescence (±1 nm) penetration, enabling high-contrast images of cell surface and single molecules and minimizing background noise cellTIRF-4Line system has integrated point FRAP optics for the first laser line, enabling FRAP and photo-switching experiments with no additional costs Learn more about the cellTIRF system

Simultaneous Multicolor TIRF

The Olympus cellTIRF system provides true simultaneous acquisition of up to four wavelengths.

Motorized angle control for each laser provides equal evanescence (±1 nm) penetration, enabling high-contrast images of cell surface and single molecules and minimizing background noise
cellTIRF-4Line system has integrated point FRAP optics for the first laser line, enabling FRAP and photo-switching experiments with no additional costs

Learn more about the cellTIRF system

Imaging Stability

The frame architecture and focus drive design of the IXplore system offer enhanced rigidity that reduces the impact of vibration and temperature. It maintains the desired Z-axis position to facilitate reliable time-lapse imaging. When combined with the TruFocus system, the IXplore TIRF microscope system can capture high-precision, time-lapse images that are aligned and in focus.

	TIRF Objectives Total internal reflection fluorescence (TIRF) is facilitated by a wide range of objectives featuring a high signal-to-noise ratio and a correction collar to adjust for cover glass thickness and temperature. Our corrected plan apochromat objectives with an NA of 1.5 help you acquire uniform high-quality images with a large field of view. Take advantage of Olympus' remarkable TIRF objective with the world’s highest NA, 1.7. As of November, 2018. According to Olympus research. Learn more about our high NA objectives

TIRF Objectives

Total internal reflection fluorescence (TIRF) is facilitated by a wide range of objectives featuring a high signal-to-noise ratio and a correction collar to adjust for cover glass thickness and temperature. Our corrected plan apochromat objectives with an NA of 1.5 help you acquire uniform high-quality images with a large field of view. Take advantage of Olympus' remarkable TIRF objective with the world’s highest NA, 1.7*.

*As of November, 2018. According to Olympus research.

Learn more about our high NA objectives

Precise and Intuitive Photomanipulation (Optional Peripherals)

Olympus’ cellFRAP photomanipulation device and real-time controller (U-RTCE) enable accurate control (200 µs dead time), diffraction-limited stimulation with a flexible region of interest, and precise reproduction of experimental conditions.

Learn more about the cellFRAP

Elixon Theatre jQuery Plugin . ,	Advanced Analysis Advanced functionality, such as object tracking, Count and Measure, Kymograph and real time 2D deconvolution to enhance object identification.

Advanced Analysis

Advanced functionality, such as object tracking, Count and Measure, Kymograph and real time 2D deconvolution to enhance object identification.

Fast, Microsecond-Accurate Devices

The fast filter wheel, shutter, LED light source control, and real-time controllers (U-RTC) enable less photobleaching and phototoxicity, resulting in healthier cells and more robust data.

Learn more about the U-RTC/E

References

Y. Yang, et al. Spectraplakin induces positive feedback between fusogens and the actin cytoskeleton to promote cell-cell fusion. Developmental Cell (April 10, 2017).

A. R. van Vliet, et al. The ER stress sensor PERK coordinates ER-plasma membrane contact site formation through interaction with filamin-A and F-actin remodeling. Molecular Cell (Feburuary 23, 2017).

F. Hertel, et al. RefSOFI for mapping nanoscale organization of protein-protein interactions in living cells. Cell Reports (December 31, 2015).

C. Cauvin, et al. Rab35 GTPase triggers switch-like recruitment of the lowe syndrome lipid phosphatase OCRL on newborn endosomes. Current Biology (December 24, 2015).

W.-K. Ji, et al. Actin filaments target the oligomeric maturation of the dynamin GTPase Drp1 to mitochondrial fission sites. eLIFE (November 26, 2015).

A. Juanes-Garcia, et al. A regulatory motif in nonmuscle myosin II-B regulates its role in migratory front–back polarity. The Journal of Cell Biology (April 13, 2015).

D. Borrenberghs, et al. HIV virions as nanoscopic test tubes for probing oligomerization of the integrase enzyme. ACS Nano (March 21, 2014).

S. Yamaoka, et al. Identification and dynamics of arabidopsis adaptor protein-2 complex and its involvement in floral organ development. The Plant Cell (August 23, 2013)

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Microscope Frame			IX83P2ZF
Observation Method	Total Internal Reflection Fluorescence		✓
Observation Method	Fluorescence (Blue/Green Excitation)		✓
Observation Method	Fluorescence (Ultraviolet Excitation)		✓
Observation Method	Differential Interference Contrast (DIC)		✓
Observation Method	Phase Contrast		✓
Observation Method	Brightfield		✓
Revolving Nosepiece	Motorized (6 position)		✓
Focus	Motorized		✓
Focus	Z Drift Compensator		✓
Observation Tubes	Widefield (FN 22)	Tilting Binocular	✓
Illuminator	Transmitted Köhler Illuminator	LED Lamp	✓
Illuminator	Transmitted Köhler Illuminator	100 W Halogen Lamp	✓
Illuminator	Fluorescence Illuminator	100 W Mercury Lamp	✓
Illuminator	Fluorescence Illuminator	Light Guide Illumination	✓
Fluorescence Mirror Turret	Motorized (8 position)		✓
Stage	Motorized		Contact your local sales representative to hear about motorized stage options
Condenser	Motorized	Universal Condenser	W.D. 27 mm, NA 0.55, motorized aperture and polarizer
Condenser	Manual	Universal Condenser	NA 0.55/ W.D. 27 mm
Condenser	Manual	Ultra-Long Working Distance Condenser	NA 0.3/ W.D. 73.3 mm
Confocal Scanner			-
Super Resolution Processing			-
Accessories			Remote correction collar controller (IX3-RCC) Real time controller (U-RTC/U-RTCE) TIRF illuminator Incubation housing
Dimensions (W × D × H)			323 (W) x 475 (D) x 706 (H) mm (IX83 microscope frame)
Weight			Approx. 47kg (IX83P2ZF)