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Inverted Microscopes

倒立顕微鏡は、実験室での細胞分析に欠かすことのできない重要な機器です。倒立顕微鏡は、高度な画像取得技術により、蛍光や明視野の観察法を用いてライフサイエンス研究目的に合った細胞観察を可能にします。

エビデントでは、ルーチン倒立顕微鏡、倒立位相差顕微鏡、倒立蛍光顕微鏡、共焦点および超解像倒立顕微鏡といった幅広いラインアップをご用意しています。正確で精密なイメージングを提供するために設計された、人間工学に基づく数々の顕微鏡からお選びください。

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IXplore Automated Inverted Microscope System

IXplore™ IX85 SpinXL

See more and discover faster with an unparalleled 26.5 mm field of view (FOV).

  • Capture more fast cellular dynamics in a single frame at imaging speeds up to 498 fps Precise, gentle live-cell imaging maintains sample viability
  • Supports a wide range of applications and users with speed, precision, and flexibility
  • Streamlines tasks throughout the lab with intelligent automation and easy workflows
  • Evolves with your research via a wide wavelength range, tailored disk options, and expandable imaging modalities
  • Powered by CrestOptics spinning disk technology and enhanced with the IXplore™ IX85 platform
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IXplore™ IX85 SpinSR

Capture ultra-small cellular dynamics in stunning super-resolution detail.

  • Discover more with high-speed imaging in resolution down to 120 nm
  • Designed for prolonged cell viability in long-term time-lapse experiments
  • Powered by Yokogawa spinning disk technology Enhanced with the IXplore™ IX85 platform and Evident’s TruSight™ SR super-resolution algorithm
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IXplore™ IX85

The IXplore™ IX85 platform delivers an unmatched level of customizability, allowing you to design or build an intelligent, high-performance imaging system that meets your specific goals. And with an industry-leading field number (FN) of 26.5mm plus an array of advanced end-to-end imaging and workflow features, the IXplore™ IX85 means you can capture more than ever before while dramatically reducing your acquisition times.

IXplore™ IX85 Live

Designed to reduce photobleaching and phototoxicity, the IXplore™ IX85 Live system is optimized for physiological experiments involving live cell and tissue observation. Offering precise environmental control and enhanced rigidity, it supports long-term cell viability and stability for time-lapse imaging applications, such as in cancer, stem cell, and brain research.

  • Maintain focus accurately and reliably in time-lapse experiments with TruFocus™ Z-drift compensation system
  • Discover the real morphology of your cells with Olympus silicone immersion optics

IXplore™ IX85 Pro

The foundation of our IXplore™ IX85 Pro system, the IXplore™ IX85 delivers an industry leading FOV and an array of advanced end-to-end imaging features, allowing you to see and capture more than ever before while dramatically reducing acquisition times. Experience exceptional speed, clarity, and reliability with the IXplore™ IX85 microscope system.

IXplore™ IX85 Spin

The IXplore™ IX85 Spin system features a spinning disk confocal unit that enables fast 3D image acquisition, a large field of view, and prolonged cell viability in time-lapse experiments. Researchers can use it to perform rapid 3D confocal imaging with high resolution and contrast at greater depths for imaging into thicker samples. The spinning disk also helps to cut down on photobleaching and phototoxicity of samples upon excitation.

  • TruFocus™ Z-drift compensation system maintains focus for each frame
  • Precise 3D imaging with improved light collection using X Line™ objectives

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倒立顕微鏡

Customizable Spinning Disk Confocal System

Crest CICERO

Transform your microscope into a user-friendly, reliable, and cost-effective spinning disk confocal system.

  • High-speed imaging across a 22 mm FOV
  • Access widefield and confocal modalities in a single device
  • Available in both LED and laser configurations, with wavelengths from 405–730 nm*

*Availability and configurations are region dependent. Contact your local Evident representative for more information.

Digital Imaging System

APX100

  • Easy-to-use, all-in-one microscope system
  • Publication-quality images in a few clicks
  • Fast, efficient data management
Compound Microscope System

IXplore IX83 Standard

Optimized for basic multicolor fluorescence imaging and routine experiments, the IXplore Standard system is easy to operate and ergonomically designed. Even with standard cell culture vessels, it captures high-quality, publication-worthy images while providing accurate and repeatable results at high magnifications. The IXplore Standard system’s simplified workflow and ease of use facilitate a wide range of standard imaging applications.

  • High repeatability and accuracy for standard imaging tasks
  • Benefit from the same optical capabilities found in high-end IXplore systems
  • Easily upgrade to encoded functionality to boost the reproducibility of experiments
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Automated Microscope System

IXplore IX83 Pro

The IXplore Pro automated system acquires panoramic, multichannel images without a complex workflow. Easing experiment setup, the Graphical Experimental Manager offers fully automated multidimensional observation capabilities. The microscope’s high-precision ultrasonic stage, automated focusing, and bright, uniform fluorescence illumination facilitate image stitching applications.  

  • Automated multidimensional observation (X, Y, Z, T, wavelength, and positions)
  • Boost your statistics with multiwell plate screening
  • Acquire fluorescence panoramic images of large samples, such as brain slices
  • Create 3D optical sections and enhance resolution with TruSight™ deconvolution
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Live Cell Imaging Microscope System

IXplore IX83 Live

Designed to reduce photobleaching and phototoxicity, the IXplore Live system is optimized for physiological experiments involving live cell and tissue observation. Offering precise environmental control and enhanced rigidity, it supports long-term cell viability and stability for time-lapse imaging applications, such as in cancer, stem cell, and brain research.

  • Maintain focus accurately and reliably in time-lapse experiments with TruFocus™ Z-drift compensation system
  • Discover the real morphology of your cells with Olympus silicone immersion optics
  • Real-time controller helps limit cell disturbance, enabling physiologically relevant data
Learn More
 
TIRF Imaging Microscope System

IXplore IX83 TIRF

For membrane dynamics, single-molecule detection, and colocalization experiments, the IXplore TIRF system enables sensitive simultaneous multicolor TIRF (total internal reflection fluorescence) imaging for up to four colors. Olympus’ cellTIRF system provides stable motorized individual laser-angle control, providing equal evanescent wave penetration for high-contrast, low noise images. Our TIRF objectives feature high SNR, high NA, and correction collars to adjust for cover glass thicknesses and temperature.

  • Exact colocalization of up to four markers thanks to individual penetration depth control
  • Take advantage of Olympus’ TIRF objective with the world's highest NA of 1.7*
  • Intuitive setup of complex experiments with the Graphical Experiment Manager (GEM), cellFRAP, and U-RTCE
* As of July 25, 2017. According to Olympus research.
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Confocal Imaging Microscope System

IXplore IX83 Spin

The IXplore Spin system features a spinning disk confocal unit that enables fast 3D image acquisition, a large field of view, and prolonged cell viability in time-lapse experiments. Researchers can use it to perform rapid 3D confocal imaging with high resolution and contrast at greater depths for imaging into thicker samples. The spinning disk also helps to cut down on photobleaching and phototoxicity of samples upon excitation.

  • Real-time controller (U-RTCE) helps optimize the device’s speed and precision during automated acquisition
  • TruFocus™ Z-drift compensation system maintains focus for each frame
  • Precise 3D imaging with improved light collection using X Line™ objectives
  • Upgrade to the IXplore SpinSR super resolution system as your research progresses
Learn More
 
Super Resolution Microscope System

IXplore IX83 SpinSR

The IXplore SpinSR system is our confocal super resolution microscope optimized for 3D imaging of live cell specimens. Like the IXplore Spin system, it has a spinning disk system for fast 3D imaging while limiting phototoxicity and bleaching. However, it achieves super resolution images down to 120 nm XY and enables you to switch between widefield, confocal, and super resolution with the click of a button.

  • Sharp, clear super resolution imaging down to 120 nm XY, owing to Olympus Super Resolution (OSR)
  • Prolonged cell viability in confocal time-lapse imaging due to less phototoxicity and bleaching
  • Use two cameras simultaneously to achieve fast, two-color super-resolution imaging
  • Super resolution imaging with the world’s first plan apochromat objectives with a numerical aperture (NA) of 1.5*
* As of November 2018. According to Olympus research.
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Compact Cell Culture Microscope

CKX53

The CKX53 microscope eases the cell and tissue culture workflow, simplifying steps such as live cell observation, cell sampling and handling, image capture, and fluorescence observation. Its integrated phase contrast system, compact, ergonomic design, and stable performance enable simple, efficient cell observation. The universal sample holder and expandable stage accommodate a wide variety of cell culture container types and sizes.

  • Precentered phase contrast
  • Inversion contrast (IVC) technique provides clear three-dimensional views
  • Fluorescence with a 3-position slider
  • View multilayer tissue flasks up to 190 mm (7.5 in.) in height up thanks to the detachable condenser

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リサーチ用倒立顕微鏡のよくあるご質問

倒立顕微鏡の仕組みを教えてください。

倒立顕微鏡は正立顕微鏡と似ていますが、逆さまの構成で部品が配置されているため、「倒立」顕微鏡と呼ばれます。正立顕微鏡では、透過光源やコンデンサーレンズがステージや標本の下にあります。倒立顕微鏡では、コンデンサーレンズや透過光源は標本の上にあり、対物レンズ、蛍光光源、ミラーターレットは標本の下にあります。

倒立顕微鏡の部品配置は、光を上から照射し、像を下から観察するためのものです。そのため、倒立顕微鏡はガラス製フラスコやペトリ皿などの細胞培養容器の底に付着したサンプルの観察に適しています。

倒立顕微鏡はどのように使用するのですか?

倒立顕微鏡では、透過光路と落射光路の両方を使用して、正確な細胞観察や解析を行うための高倍率レンズを使用します。多くの倒立顕微鏡には固定ステージがあり、サンプルにピントを合わせるために高倍率対物レンズを垂直軸に沿って動かせます(サンプルをレンズに近づけたり離したりできます)。ピントを合わせたら、オープンフレーム型顕微鏡の接眼レンズでサンプルを観察することも、カメラ付き倒立顕微鏡を使用する場合はPC画面で観察することもできます。倒立顕微鏡の対物レンズにイマージョンオイルを塗布する方法と、サンプルをステージにセットする方法について、以下のビデオをご覧ください。

倒立蛍光顕微鏡とは何ですか?

前述のように、倒立顕微鏡では、透過光源とコンデンサーレンズがステージの上に下向きに配置され、対物レンズやターレットはステージの下に上向きに配置されています。IXplore™ StandardIXplore Proシステムなどのオープンフレーム型倒立顕微鏡は、ニーズに応じてシステムの適合やカスタマイズを考えている研究室に最適なソリューションです。TIRF照明やスピニングディスク型共焦点モジュールなどのマルチモーダルソリューションと連動し、柔軟なイメージングオプションを備えています。通常、蛍光イメージングにオープンフレーム型倒立顕微鏡を使用する場合は、室内光からの干渉信号を避けるため、専用の暗室が必要になります。

専用の暗室を用意できない場合は、密閉式のオールインワン倒立蛍光顕微鏡(APEXVIEW™ APX100デジタルイメージングシステムなど)が最良の選択肢です。オールインワン倒立蛍光顕微鏡はボックス型フレームを持つ自動リサーチ顕微鏡で、通常の照明条件によるイメージングが可能です。高度な蛍光システムや共焦点システムの設定にありがちな複雑な手順が自動ワークフローによって軽減され、コンパクトで多用途に対応するこの倒立顕微鏡は、研究室やコアファシリティのほぼどこにでも設置できます。

倒立顕微鏡に関連するビデオ資料

倒立型リサーチ顕微鏡IXploreシリーズ

研究者のニーズに応じて最適なシステムを、倒立顕微鏡IX3をベースにIXploreシリーズとしてラインアップしました。 蛍光ドキュメンテーションからライブセル、共焦点イメージングや超解像まで目的に応じたイメージングシステムをIXploreシリーズよりお選びいただけます。

倒立顕微鏡へのサンプルの設置

このビデオでは、標準の1×3インチスライドを使ってIXplore倒立顕微鏡システムにサンプルを載せる方法を紹介しています。

生細胞イメージング用シリコーンオイル浸対物レンズ

生細胞を観察するとき、適切な対物レンズを使えば鮮明に見ることができます。このビデオでは、細胞や組織観察の際に、シリコーンオイル浸対物レンズで高精度かつ高分解能の画像を得る方法をご覧いただけます。

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