Two software products can be used for length measurement:
To confirm that the measurement settings are correct, youʼll need a 0.01 μm stage micrometer from Evident or a third party. These can be purchased from Evident.
Evident: Stage micrometer
Model: OB-M1/100micro
Step 1: Set the objective micrometer on the microscopeʼs stage.
Step 2: Move the stage so that the micrometerʼs scales are in the center of the screen. Make sure theyʼre in focus.

*Image shown is from cellSens software
Step 3: Check that the magnification of the objective youʼre using is correctly selected in the software.

cellSens standard layout
cellSens simple layout
Stand-alone camera software (DP2-SAL) |
Stand-alone camera software (DP2-AOU) |
Step 4: Measure using the scale function and check that the results are correct.
✓ 100x objective: 50 µm (5 divisions)
✓ Objectives other than 100x: 100 µm (10 divisions)

Note: For details about the stand-alone connection kit (DP2-SAL/DP2-AOU) measurement method, please refer to the softwareʼs instruction manual.
Step 5: Repeat step 4 for the objectives you are going to use.
*When you change objectives, be sure to verify the settings (see step 3).
If the measurement results differ from what you expect, thereʼs a possibility that the magnification setting is not correctly specified. Please check the magnification setting and change it if itʼs not correct.
If you have any questions, please contact your local Evident sales representative or our customer support center.
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