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A log phase culture of Vero cells was fixed, permeabilized, blocked, and treated with mouse anti-C-protein (myocardial) monoclonal primary antibodies followed by goat anti-mouse secondary antibodies conjugated to Alexa Fluor 568. Mixed in with the secondary reagent was Alexa Fluor 488 conjugated to phalloidin to label the filamentous actin cytoskeleton. Nuclei were counterstained with Hoechst 33258.
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