In order to label the intermediate filaments in the log phase adherent FoLu culture shown in this confocal image, the fixed and permeabilized cells were blocked and treated with mouse anti-vimentin (porcine eye lens) primary antibodies followed by goat anti-mouse secondary antibodies (IgG) conjugated to Alexa Fluor 488. Cell nuclei were counterstained with the red-absorbing dye TO-PRO-3.
对不起,此内容在您的国家不适用。